Saito Takeshi, Abe Daigo, Sekiya Keizo
National Agricultural Research Center for Western Region, 1-3-1 Senyu-cho, Zentsuji 765-8508, Japan.
Biochem Biophys Res Commun. 2009 Mar 6;380(2):281-5. doi: 10.1016/j.bbrc.2009.01.058. Epub 2009 Jan 22.
Flavanones are class of polyphenolic compounds, some of which are found in foods and provide health benefits. In this study, we show that flavanone significantly enhances differentiation of 3T3-L1 preadipocytes. During adipogenesis, flavanone enhanced expression of genes and accumulation of proteins that are involved in adipocyte function. Some reports have indicated that flavanone inhibits proliferation of mammalian cells, and down-regulates expression of growth-related proteins. Such proteins include phosphorylated ERK1/2, cyclins, and Cdks that are important for an early event in adipogenesis, mitotic clonal expansion (MCE). We demonstrated that flavanone did not inhibit MCE or expression of MCE-related proteins, except for a modest inhibition of cyclin D1 expression. Using luciferase reporter assays, we found that flavanone acted as a peroxisome proliferator-activated receptor gamma (PPARgamma) ligand in a dose-dependent manner. Together, our results suggest that flavanone enhances adipogenesis, at least in part, through its PPARgamma ligand activity.
黄烷酮是一类多酚化合物，其中一些存在于食物中并具有健康益处。在本研究中，我们表明黄烷酮可显著增强3T3-L1前脂肪细胞的分化。在脂肪生成过程中，黄烷酮增强了与脂肪细胞功能相关的基因表达和蛋白质积累。一些报告表明，黄烷酮可抑制哺乳动物细胞的增殖，并下调与生长相关的蛋白质的表达。这些蛋白质包括对脂肪生成早期事件——有丝分裂克隆扩增（MCE）很重要的磷酸化ERK1/2、细胞周期蛋白和周期蛋白依赖性激酶。我们证明，黄烷酮除了对细胞周期蛋白D1的表达有适度抑制外，并不抑制MCE或与MCE相关的蛋白质的表达。使用荧光素酶报告基因检测，我们发现黄烷酮以剂量依赖性方式作为过氧化物酶体增殖物激活受体γ（PPARγ）配体发挥作用。总之，我们的结果表明，黄烷酮至少部分通过其PPARγ配体活性增强脂肪生成。
