一种制备分散胰腺腺泡的新的快速方法。
A new, rapid, method for preparation of dispersed pancreatic acini.
作者信息
Bruzzone R, Halban P A, Gjinovci A, Trimble E R
出版信息
Biochem J. 1985 Mar 1;226(2):621-4. doi: 10.1042/bj2260621.
Abstract
A new method for the preparation of pancreatic acini is described. The method is simple and much more rapid than previously described techniques, the time required for preparation of pancreatic acini being 20 min from removal of the pancreas. Acini prepared with this method perform in a superior manner when stimulated by either caerulein or secretin. Thus this new technique would be ideal for use in binding and secretion studies.
摘要
本文描述了一种制备胰腺腺泡的新方法。该方法简单，比先前描述的技术要快得多，从取出胰腺开始，制备胰腺腺泡所需的时间为20分钟。用这种方法制备的腺泡在受到蛙皮素或促胰液素刺激时表现更优。因此，这项新技术将非常适合用于结合和分泌研究。
相似文献
1
A new, rapid, method for preparation of dispersed pancreatic acini.一种制备分散胰腺腺泡的新的快速方法。
Biochem J. 1985 Mar 1;226(2):621-4. doi: 10.1042/bj2260621.
2
Electrophoretic and cytological evidence for heterogeneity of pancreatic acinar cell responsiveness to carbachol, caerulein and secretin.胰腺腺泡细胞对卡巴胆碱、蛙皮素和促胰液素反应性异质性的电泳及细胞学证据。
Cell Tissue Res. 1985;239(1):105-9. doi: 10.1007/BF00214909.
3
Neurotensin interacts with carbachol, secretin, and caerulein in the stimulation of the exocrine pancreas of the rat in vitro.在体外刺激大鼠外分泌胰腺方面，神经降压素与卡巴胆碱、促胰液素和蛙皮素相互作用。
Regul Pept. 1983 Oct;7(2):137-43. doi: 10.1016/0167-0115(83)90305-1.
4
Influence of secretin on caerulein stimulated pancreatic secretion of the rat.促胰液素对蛙皮素刺激的大鼠胰腺分泌的影响。
Pharmacol Res Commun. 1977 Jul;9(7):633-41. doi: 10.1016/s0031-6989(77)80093-3.
5
Effects of secretin and caerulein on enzymes of cultured pancreatic acinar cells.促胰液素和蛙皮素对培养的胰腺腺泡细胞中酶的影响。
In Vitro Cell Dev Biol. 1991 Aug;27A(8):660-4. doi: 10.1007/BF02631110.
6
Rapid increases in inositol 1,4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate and cytosolic free Ca2+ in agonist-stimulated pancreatic acini of the rat. Effect of carbachol, caerulein and secretin.大鼠激动剂刺激的胰腺腺泡中肌醇1,4,5-三磷酸、肌醇1,3,4,5-四磷酸和胞质游离Ca2+的快速增加。卡巴胆碱、蛙皮素和促胰液素的作用。
Biochem J. 1987 Feb 15;242(1):289-92. doi: 10.1042/bj2420289.
7
Comparative effects of CCK receptor antagonists on rat pancreatic secretion in vivo.
Am J Physiol. 1989 Jan;256(1 Pt 1):G150-7. doi: 10.1152/ajpgi.1989.256.1.G150.
8
Action of secretagogues on a new preparation of functionally intact, isolated pancreatic acini.促分泌素对一种新制备的功能完整的离体胰腺腺泡的作用。
Am J Physiol. 1978 Nov;235(5):517-24. doi: 10.1152/ajpendo.1978.235.5.E517.
9
Modifications in pancreatic enzyme proportions following secretory stimulations.分泌刺激后胰腺酶比例的变化。
Digestion. 1984;29(3):152-7. doi: 10.1159/000199025.
10
Caerulein causes translocation of protein kinase C in rat acini without increasing cytosolic free Ca2+.
Am J Physiol. 1988 Jul;255(1 Pt 1):G33-9. doi: 10.1152/ajpgi.1988.255.1.G33.
引用本文的文献
1
secretion of zymogens by bovine pancreatic acini and ultra-structural analysis of exocytosis.牛胰腺腺泡的酶原分泌及胞吐作用的超微结构分析
Biochem Biophys Rep. 2015 Dec 23;5:237-245. doi: 10.1016/j.bbrep.2015.12.009. eCollection 2016 Mar.
2
Mitochondrial dysfunction and apoptosis of acinar cells in chronic pancreatitis.慢性胰腺炎中腺泡细胞的线粒体功能障碍与凋亡
J Gastroenterol. 2008;43(6):473-83. doi: 10.1007/s00535-008-2179-4. Epub 2008 Jul 4.
3
Primary culture of pancreatic (human) acinar cells.胰腺（人）腺泡细胞的原代培养。
Dig Dis Sci. 2008 Sep;53(9):2569-75. doi: 10.1007/s10620-007-0162-1. Epub 2008 Feb 2.
4
Protein tyrosine phosphatase kappa and SHP-1 are involved in the regulation of cell-cell contacts at adherens junctions in the exocrine pancreas.蛋白酪氨酸磷酸酶κ和SHP-1参与外分泌胰腺中黏附连接的细胞间接触调节。
Gut. 2005 Oct;54(10):1445-55. doi: 10.1136/gut.2004.063164. Epub 2005 Jun 29.
5
Multiple isoforms of the ryanodine receptor are expressed in rat pancreatic acinar cells.大鼠胰腺腺泡细胞中表达了兰尼碱受体的多种亚型。
Biochem J. 2000 Oct 1;351(Pt 1):265-71. doi: 10.1042/0264-6021:3510265.
6
Actin coating of secretory granules during regulated exocytosis correlates with the release of rab3D.在调节性胞吐过程中，分泌颗粒的肌动蛋白包被与rab3D的释放相关。
Proc Natl Acad Sci U S A. 2000 Feb 1;97(3):1091-5. doi: 10.1073/pnas.97.3.1091.
7
A membrane lipid imbalance plays a role in the phenotypic expression of cystic fibrosis in cftr(-/-) mice.膜脂质失衡在cftr(-/-)小鼠囊性纤维化的表型表达中起作用。
Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13995-4000. doi: 10.1073/pnas.96.24.13995.
8
On the protective mechanisms of nitric oxide in acute pancreatitis.一氧化氮在急性胰腺炎中的保护机制
Gut. 1998 Sep;43(3):401-7. doi: 10.1136/gut.43.3.401.
9
Enhanced secretion of amylase from exocrine pancreas of connexin32-deficient mice.连接蛋白32缺陷型小鼠外分泌胰腺淀粉酶分泌增强。
J Cell Biol. 1998 Jun 1;141(5):1267-75. doi: 10.1083/jcb.141.5.1267.
10
Elevated calcium and activation of trypsinogen in rat pancreatic acini.大鼠胰腺腺泡中钙升高及胰蛋白酶原激活。
Gut. 1997 Sep;41(3):339-43. doi: 10.1136/gut.41.3.339.
本文引用的文献
1
Calcium uptake into acini from rat pancreas: evidence for intracellular ATP-dependent calcium sequestration.大鼠胰腺腺泡对钙的摄取：细胞内ATP依赖的钙螯合的证据。
J Membr Biol. 1982;65(3):205-20. doi: 10.1007/BF01869964.
2
Regulatory mechanisms in pancreas and salivary acini.胰腺和唾液腺腺泡中的调节机制。
Annu Rev Physiol. 1984;46:361-75. doi: 10.1146/annurev.ph.46.030184.002045.
3
Identification and characterization of receptors for secretagogues on pancreatic acinar cells.胰腺腺泡细胞上促分泌素受体的鉴定与特性分析。
Fed Proc. 1981 Aug;40(10):2486-96.
4
Interaction of cholecystokinin with specific membrane receptors on pancreatic acinar cells.胆囊收缩素与胰腺腺泡细胞上特定膜受体的相互作用。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2079-83. doi: 10.1073/pnas.77.4.2079.
5
Glucose-insulin interactions on exocrine secretion from the perfused rat pancreas.灌注大鼠胰腺外分泌中葡萄糖与胰岛素的相互作用
Gastroenterology. 1984 Dec;87(6):1305-12.
6
Receptors for vasoactive intestinal peptide and secretin on rat pancreatic acini.
Am J Physiol. 1984 Jun;246(6 Pt 1):G710-7. doi: 10.1152/ajpgi.1984.246.6.G710.
7
Intracellular free calcium concentrations in isolated pancreatic acini; effects of secretagogues.分离的胰腺腺泡细胞内游离钙浓度；促分泌剂的作用
Biochem Biophys Res Commun. 1983 Nov 30;117(1):122-8. doi: 10.1016/0006-291x(83)91549-8.
8
Postreceptor modulation of action of VIP and secretin on pancreatic enzyme secretion by secretagogues that mobilize cellular calcium.通过动员细胞钙的促分泌素对血管活性肠肽和促胰液素作用于胰腺酶分泌的受体后调节。
Am J Physiol. 1982 Apr;242(4):G423-8. doi: 10.1152/ajpgi.1982.242.4.G423.
9
A rat pancreas-small gut preparation for the study of intestinal factor(s) and insulin release.一种用于研究肠因子和胰岛素释放的大鼠胰腺-小肠制备物。
Diabetes. 1969 Nov;18(11):733-8. doi: 10.2337/diab.18.11.733.
10
Method for the isolation of intact islets of Langerhans from the rat pancreas.从大鼠胰腺中分离完整胰岛的方法。
Diabetes. 1967 Jan;16(1):35-9. doi: 10.2337/diab.16.1.35.
Zotero 插件