Zhang Yuqin, Zhang Shengnan, Li Huang, Huang Mei, Xu Wei, Chu Kedan, Chen Lidian, Chen Xianwen
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.
College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.
Mol Med Rep. 2015 Jul;12(1):988-94. doi: 10.3892/mmr.2015.3515. Epub 2015 Mar 19.
Gualou Guizhi decoction (GLGZD) is a well-established Traditional Chinese Medicinal formulation which has long been used to treat stroke in a clinical setting in China. The present study investigated the ameliorative effects of GLGZD on inflammation in focal cerebral ischemic-reperfusion injury. A rat model of middle cerebral artery occlusion (MCAO) was employed. Rats were administrated GLGZD (7.2 and 14.4 g/kg per day) or saline as control 2 h after reperfusion and daily over the following seven days. Neurological deficit score and screen test were evaluated at 1, 3, 5 and 7 days after MCAO. Brain infarct size and brain histological changes were observed via 2,3,5-triphenyltetrazolium chloride staining and regular hematoxylin & eosin staining. Furthermore, inflammation mediators and nuclear factor-κB (NF-κB) were investigated using ELISA and immunohistochemistry. GLGZD treatment significantly improved neurological function, ameliorated histological changes to the brain and decreased infarct size in focal cerebral ischemic-reperfusion injury. GLGZD was found to significantly reduce interleukin (IL)-1, tumor necrosis factor-α and NF-κB levels, while increasing levels of IL-10. In conclusion, the present study suggested that GLGZD has a neuroprotective effect on focal cerebral ischemic-reperfusion injury and this effect is likely to be associated with the anti-inflammatory function of GLGZD.
瓜蒌桂枝汤（GLGZD）是一种成熟的中药配方，在中国临床上长期用于治疗中风。本研究探讨了瓜蒌桂枝汤对局灶性脑缺血再灌注损伤炎症的改善作用。采用大脑中动脉闭塞（MCAO）大鼠模型。大鼠在再灌注后2小时给予瓜蒌桂枝汤（每天7.2和14.4克/千克）或生理盐水作为对照，并在接下来的七天内每日给药。在MCAO后1、3、5和7天评估神经功能缺损评分和筛选试验。通过2,3,5-三苯基四氮唑氯化物染色和常规苏木精和伊红染色观察脑梗死体积和脑组织结构变化。此外，使用酶联免疫吸附测定（ELISA）和免疫组织化学研究炎症介质和核因子-κB（NF-κB）。瓜蒌桂枝汤治疗显著改善了局灶性脑缺血再灌注损伤的神经功能，改善了脑组织学变化并减小了梗死体积。发现瓜蒌桂枝汤可显著降低白细胞介素（IL）-1、肿瘤坏死因子-α和NF-κB水平，同时提高IL-10水平。总之，本研究表明瓜蒌桂枝汤对局灶性脑缺血再灌注损伤具有神经保护作用，且这种作用可能与瓜蒌桂枝汤的抗炎功能有关。
