Sun Chengtao, Xu Wen, Zhang Yuqin, Yu Lishuang, Ye Miao, Chu Kedan, Xu Wei, Lin Yu
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China.
Centre of Biomedical Research & Development, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China.
J Anal Methods Chem. 2017;2017:8451383. doi: 10.1155/2017/8451383. Epub 2017 Jul 20.
A rapid and sensitive ultrafast performance liquid chromatography-tandem mass spectrometry method (UPLC-MS/MS) was developed for the simultaneous determination of 11 compounds in Gualou Guizhi Granule (GLGZG), including liquiritin, isoliquiritin, liquirtin apioside, isoliquiritin apioside, liquiritigenin, isoliquiritigenin, glycyrrhizic acid, glycyrrhetinic acid, paeoniflorin, albiflorin, and paeoniflorin sulfonate in rat plasma. UPLC-MS/MS assay with negative ion mode was performed on a Waters CORTECS C18 (2.1 × 100 mm, 1.6 m) with the mobile phase consisting of 0.1% aqueous formic acid (A) and acetonitrile (B) in gradient elution at a flow rate of 0.25 mL·min. The method was linear for all analytes within the detection range ( ≥ 0.9597). The inter- and intraday precision (RSD) were 2.21-6.41% and 1.67-6.18%; the inter- and intraday accuracy (recover) were 92.48-114.03% and 90.23-112.04%. And the recovery rate ranged from 81.30% to 108.22%. The matrix effect values obtained for analytes ranged from 88.91% to 113.32%. This validated method was successfully applied to a pharmacokinetics study in rats after oral administration of GLGZG.
建立了一种快速灵敏的超快速液相色谱-串联质谱法（UPLC-MS/MS），用于同时测定大鼠血浆中瓜蒌桂枝颗粒（GLGZG）中的11种化合物，包括甘草苷、异甘草苷、甘草苷元芹糖甙、异甘草苷元芹糖甙、甘草素、异甘草素、甘草酸、甘草次酸、芍药苷、芍药内酯苷和芍药苷磺酸盐。采用负离子模式的UPLC-MS/MS法在Waters CORTECS C18（2.1×100 mm，1.6 μm）色谱柱上进行，流动相由0.1%甲酸水溶液（A）和乙腈（B）组成，梯度洗脱，流速为0.25 mL·min。该方法在检测范围内对所有分析物均呈线性（≥0.9597）。日间和日内精密度（RSD）分别为2.21 - 6.41%和1.67 - 6.18%；日间和日内准确度（回收率）分别为92.48 - 114.03%和90.23 - 112.04%。回收率范围为81.30%至108.22%。分析物的基质效应值范围为88.91%至113.32%。该验证方法成功应用于大鼠口服GLGZG后的药代动力学研究。
