Fitzgerald M, Wall P D, Goedert M, Emson P C
Brain Res. 1985 Apr 15;332(1):131-41. doi: 10.1016/0006-8993(85)90396-8.
The sciatic nerve was sectioned unilaterally in rats and nerve growth factor (NGF) applied locally to the nerve stump for the following 10-14 days using an indwelling osmotic pump. The aim of the experiment was to test whether NGF had any effect on the previously reported neurophysiological and neurochemical events that occur central to a peripheral nerve lesion. The method of application allowed the sciatic nerve on the other side to be used as a control. Primary afferent depolarization fell, as expected, to 13% of its control value after chronic nerve section but if NGF was administered it fell to only 43.5% of control. Chronic nerve section is also known to result in expansion of the receptive fields of deafferented dorsal horn cells. NGF treatment reduced the number of such large receptive fields by 50%. The normal depletion of fluoride resistant acid phosphatase from the cut nerve terminals in the dorsal horn did not occur following NGF treatment. Radioimmunoassay of substance P revealed that the 30% reduction in dorsal horn levels that follows chronic sciatic nerve section did not occur when NGF was applied and that the accompanying 60% decrease in dorsal root ganglion levels was changed to a 64% increase by NGF. The results show that chronic NGF treatment of a cut sciatic nerve does partially reverse the central changes that normally follow deafferentation.
在大鼠中单侧切断坐骨神经，并使用植入式渗透泵在接下来的10 - 14天内将神经生长因子（NGF）局部应用于神经残端。该实验的目的是测试NGF是否对先前报道的发生在周围神经损伤中枢的神经生理和神经化学事件有任何影响。应用方法允许将另一侧的坐骨神经用作对照。如预期的那样，慢性神经切断后，初级传入去极化降至其对照值的13%，但如果给予NGF，其仅降至对照值的43.5%。慢性神经切断还已知会导致去传入背角细胞的感受野扩大。NGF治疗使此类大感受野的数量减少了50%。NGF治疗后，背角中切断的神经末梢正常的耐氟酸性磷酸酶消耗未发生。对P物质的放射免疫分析显示，慢性坐骨神经切断后背角水平降低30%，而应用NGF时未出现这种情况，并且背根神经节水平伴随的60%降低被NGF改变为64%的增加。结果表明，对切断的坐骨神经进行慢性NGF治疗确实部分逆转了通常伴随去传入而发生的中枢变化。
