[医院获得性铜绿假单胞菌菌株中细胞毒性效应分子的流行率]

[Prevalence of cytotoxicity effectors in nosocomial Pseudomonas Aeruginosa strains].

作者信息

Kuznetsova M V, Maksimova A V, Karpunina T I, Demakov V A

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 2014 Nov-Dec(6):9-14.

PMID:25816507

Abstract

AIM

Analysis of occurrence of the third type secretory system (TTSS) effectors in clinical P. aeruginosa strains.

MATERIALS AND METHODS

Intra-hospital (n = 164) and extra-hospital (n = 30) strains of P. aeruginosa were studied. Detection of exoS and exoU genes was carried out by PCR in DNA Engine Dyad Thermal Cycler ("Bio-Rad", USA). Metallo-beta-lactamase (MBL) producers were detected by the presence of blaVIM-2 gene.

RESULTS

Screening of intra- and extra-hospital strains for the presence of genes coding ExoS and ExoU showed, that exoS is detected in genome of clinical isolates in 59.8% and exoU--31.1% of cases. At the same time, strains with exoS-/exoU+ genotype predominated in lCU (Φ = 0.466; p = 0.0000). A significant association between the presence of the respective effectors and material of strain isolation was not detected. exoU gene was more frequently detected in genome of MBL producers (Φ = 0.784; p = 0.0004).

CONCLUSION

A significant association between exoU and blaVIM-2 could be explained by clonal prevalence of P. aeruginosa ST235 VIM-2, circulation of those is noted on all the territory of Russia. As a rule, ExoU is produced by highly virulent poly-antibiotic resistant hospital isolates that determine unfavorable outcomes of pseudomonas infection.

摘要

目的

分析临床铜绿假单胞菌菌株中Ⅲ型分泌系统（TTSS）效应蛋白的发生情况。

材料与方法

研究了医院内（n = 164）和医院外（n = 30）的铜绿假单胞菌菌株。采用美国“伯乐”公司的DNA Engine Dyad Thermal Cycler通过PCR检测exoS和exoU基因。通过blaVIM - 2基因的存在来检测金属β - 内酰胺酶（MBL）产生菌。

结果

对医院内和医院外菌株进行编码ExoS和ExoU基因的筛查显示，临床分离株基因组中exoS的检出率为59.8%，exoU的检出率为31.1%。同时，exoS⁻/exoU⁺基因型菌株在重症监护病房中占主导地位（Φ = 0.466；p = 0.0000）。未检测到相应效应蛋白的存在与菌株分离材料之间存在显著关联。exoU基因在MBL产生菌的基因组中更频繁地被检测到（Φ = 0.784；p = 0.0004）。