Kubota Y, Takagi H, Morishima Y, Powell J F, Smith A D
Brain Res. 1985 Apr 29;333(1):188-92. doi: 10.1016/0006-8993(85)90145-3.
The 'mirror technique' was applied in immunoelectron microscopy to demonstrate the synaptic relationship between neuronal structures containing catecholamines and substance P in the caudal part of nucleus of the solitary tract in the rat, using antisera against tyrosine hydroxylase and substance P. Substance P-immunoreactive axon terminals were shown to make two types of synaptic contacts (asymmetrical and symmetrical) with catecholaminergic neurons. It is concluded that substance P afferents can directly affect catecholaminergic neurons in this nucleus via synapses.
采用抗酪氨酸羟化酶和P物质的抗血清，运用“镜像技术”进行免疫电子显微镜检查，以显示大鼠孤束核尾部含儿茶酚胺的神经元结构与P物质之间的突触关系。结果显示，P物质免疫反应性轴突终末与儿茶酚胺能神经元形成两种类型的突触联系（不对称型和对称型）。得出的结论是，P物质传入神经可通过突触直接影响该核团中的儿茶酚胺能神经元。
