在胶质瘤研究中使用生物发光成像。

Using bioluminescence imaging in glioma research.

作者信息

Luwor Rodney B, Stylli Stanley S, Kaye Andrew H

机构信息

Department of Surgery, Level 5, Clinical Sciences Building, The University of Melbourne, The Royal Melbourne Hospital, Grattan Street, Parkville, VIC 3050, Australia.

Department of Surgery, Level 5, Clinical Sciences Building, The University of Melbourne, The Royal Melbourne Hospital, Grattan Street, Parkville, VIC 3050, Australia; Department of Neurosurgery, The Royal Melbourne Hospital, Parkville, VIC, Australia.

出版信息

J Clin Neurosci. 2015 May;22(5):779-84. doi: 10.1016/j.jocn.2014.11.001. Epub 2015 Mar 26.

DOI:10.1016/j.jocn.2014.11.001

PMID:25816780

Abstract

Glioblastoma multiforme (GBM) is the most common malignant brain tumour and has the worst prognosis. Over the last decade, the use of bioluminescence imaging technology has rapidly become widespread to further understand the mechanisms that drive GBM development and progression. Pre-clinical evaluation and optimisation of therapeutic efficacy in GBM research has also utilised this simple non-invasive technology. Here we summarise recent advances made in glioma biology and therapeutic intervention using bioluminescence imaging. This review also describes the current knowledge regarding the use of luciferase-based reporters in examining the role of specific cancer signalling cascades that promote glioma progression.

摘要

多形性胶质母细胞瘤（GBM）是最常见的恶性脑肿瘤，预后最差。在过去十年中，生物发光成像技术的应用迅速普及，以进一步了解驱动GBM发生发展的机制。GBM研究中的临床前治疗效果评估和优化也采用了这种简单的非侵入性技术。在此，我们总结了利用生物发光成像在胶质瘤生物学和治疗干预方面取得的最新进展。本综述还描述了目前关于使用基于荧光素酶的报告基因来研究促进胶质瘤进展的特定癌症信号级联作用的相关知识。