Samal Juhi, Hoban Deirdre B, Naughton Carol, Concannon Ruth, Dowd Eilis, Pandit Abhay
Network of Excellence for Functional Biomaterials.
Nanomedicine (Lond). 2015;10(5):765-83. doi: 10.2217/nnm.14.221.
The in vivo therapeutic potential of neurotrophic factors to modify neuronal dysfunctions is limited by their short half-life. A biomaterials-based intervention, which protects these factors and allows a controlled release, is required.
MATERIALS & METHODS: Hollow fibrin microspheres were fabricated by charge manipulation using polystyrene templates and were loaded with NGF. Bioactivity of released NGF was demonstrated by neuronal outgrowth assay in PC-12 cells followed by in vivo assessment for NGF release and host response.
Fibrin-based hollow spheres showed high loading efficiency (>80%). Neurotrophin encapsulation into the microspheres did not alter its bioactivity and controlled release of NGF was observed in the in vivo study.
Fibrin hollow microspheres act as a suitable delivery platform for neurotrophic factors with tunable loading efficiency and maintaining their bioactive form after release in vivo.
神经营养因子改善神经元功能障碍的体内治疗潜力受其短半衰期限制。需要一种基于生物材料的干预措施来保护这些因子并实现可控释放。
使用聚苯乙烯模板通过电荷操纵制备中空纤维蛋白微球，并装载神经生长因子（NGF）。通过PC-12细胞中的神经元生长试验证明释放的NGF的生物活性，随后进行体内NGF释放和宿主反应评估。
基于纤维蛋白的中空球体显示出高负载效率（>80%）。神经营养因子包封入微球不会改变其生物活性，并且在体内研究中观察到NGF的可控释放。
纤维蛋白中空微球可作为神经营养因子的合适递送平台，具有可调节的负载效率，并在体内释放后保持其生物活性形式。
