具有KL25荚膜生物合成基因座的鲍曼不动杆菌AB5075荚膜多糖的结构解析
Structure elucidation of the capsular polysaccharide of Acinetobacter baumannii AB5075 having the KL25 capsule biosynthesis locus.
作者信息
Senchenkova Sof'ya N, Shashkov Alexander S, Popova Anastasiya V, Shneider Mikhail M, Arbatsky Nikolay P, Miroshnikov Konstantin A, Volozhantsev Nikolay V, Knirel Yuriy A
机构信息
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia.
出版信息
Carbohydr Res. 2015 May 18;408:8-11. doi: 10.1016/j.carres.2015.02.011. Epub 2015 Mar 5.
Capsular polysaccharide was isolated by the phenol-water extraction of Acinetobacter baumannii AB5075 and studied by 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the linear trisaccharide repeating unit was established: → 3)-β-D-ManpNAcA-(1 → 4)-β-D-ManpNAcA-(1 → 3)-α-D-QuipNAc4NR-(1 → where R indicates (S)-3-hydroxybutanoyl or acetyl in the ratio ∼ 2.5:1. The genes in the polysaccharide biosynthesis locus designated KL25 are appropriate to the established CPS structure.
通过对鲍曼不动杆菌AB5075进行苯酚-水提取，分离出荚膜多糖，并采用一维和二维（1）H和（13）C NMR光谱进行研究。确定了线性三糖重复单元的以下结构：→3)-β-D-甘露糖胺聚糖醛酸-(1→4)-β-D-甘露糖胺聚糖醛酸-(1→3)-α-D-喹诺糖胺4NR-(1→，其中R表示比例约为2.5:1的（S）-3-羟基丁酰基或乙酰基。多糖生物合成位点中命名为KL25的基因与所确定的CPS结构相符。
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