Zheng J, Guo N, Xi Y, Li H-Q, Zhang H, Ji L-H, Cui S, Li Z-Q
Zhang Qiu Ren Min Hospital Department of Chemotherapy for tumor Zhangqiu China.
Qinghai University Affiliated Hospital Department of Hematology Xining China linhuajicn@163.com.
Cell Mol Biol (Noisy-le-grand). 2015 Feb 28;61(1):15-9.
The aim of this study was to isolate and culture bone marrow mesenchymal stem cells (MSCs) from patients with high altitude polycythemia (HAPC) in order to provide a foundation for further exploration of their biological characteristics. MSCs were isolated and cultured from 10 HAPC patients and 10 healthy controls by using a density gradient centrifugation and an adherent screening method. The morphous of MSCs were observed under an inverted microscope, and its surface antigens were determined using flow cytometry. The growth of the MSCs was also detected to evaluate its proliferation. Bone marrow mononuclear cells were isolated from the bone marrow using a density gradient centrifugation, and they were cultured in vitro. The bone marrow MSCs were successfully isolated and cultured, which presented as fusiform and adherent cells. The MSCs in both groups expressed CD90,CD44,CD29,CD105, CD106, CD146, CD166,Stro—1 and CD13, but they did not express CD45, CD4,CD8,CD19,CD20,CD80,CD14,CD3,CD34 or HLA—DR (P>0.05). The bone marrow MSCs from HAPC patients had a higher proliferation than the bone marrow MSCs from the healthy controls (P<0.01). The bone marrow MSCs from HAPC patients can be effectively cultured in vitro.
本研究旨在从高原红细胞增多症（HAPC）患者中分离培养骨髓间充质干细胞（MSCs），为进一步探究其生物学特性奠定基础。采用密度梯度离心法和贴壁筛选法，从10例HAPC患者和10例健康对照者中分离培养MSCs。在倒置显微镜下观察MSCs的形态，采用流式细胞术检测其表面抗原。检测MSCs的生长情况以评估其增殖能力。利用密度梯度离心法从骨髓中分离出骨髓单个核细胞，并进行体外培养。成功分离培养出骨髓MSCs，其呈梭形且贴壁生长。两组MSCs均表达CD90、CD44、CD29、CD105、CD106、CD146、CD166、Stro-1和CD13，但不表达CD45、CD4、CD8、CD19、CD20、CD80、CD14、CD3、CD34或HLA-DR（P>0.05）。HAPC患者的骨髓MSCs增殖能力高于健康对照者的骨髓MSCs（P<0.01）。HAPC患者的骨髓MSCs可在体外有效培养。
