Shabanipour S, Mashayekhi F, Bahadori M H, Soruri Z Z
University of Guilan Department of Biology, Faculty of Sciences Rasht Iran.
University of Guilan Department of Biology, Faculty of Sciences Rasht Iran mashayekhi@guilan.ac.ir.
Cell Mol Biol (Noisy-le-grand). 2015 Mar 28;61(1):64-7.
Angiogenesis, invasion and decidualization play an important role in implantation and embryo development. Matrix metalloproteinases (MMPs) are crucial for the degradation/remodeling of the extracellular matrix, and are involved in spiral artery formation and invasion of endometrium during implantation. A functional single—nucleotide polymorphism (SNP) in the MMP9 promoter, —1562C/T, is known to influence gene expression in allele—specific manner. The present study evaluated the association between maternal genotype of SNP —1562C/T of MMP9 and in vitro fertilization and embryo transfer (IVF—ET) outcome in infertile women. This case-control study was comprised of infertile patients (n= 123) and women having one healthy child as controls (n= 147). Genotyping for SNP—1562C/T was performed by PCR/RFLP. Allele and genotype distribution did not differ significantly between patients and controls (P>0.05). The MMP9 genotype frequencies amongst the 123 cases were C/C=73.17%, C/T=24.40% and T/T=2.43%; the C and T allele frequencies were 85.36% and 14.63%, respectively. The MMP9 genotype frequencies amongst the 147 controls were C/C=73.46%, C/T=20.40% and T/T=6.12%; the C and T allele frequencies were 83.67% and 16.32%, respectively. In conclusion, the results of this study indicate that SNP —1562C/T of MMP9 may not be associated with IVF—ET outcome in this population.
血管生成、侵袭和蜕膜化在着床和胚胎发育过程中发挥着重要作用。基质金属蛋白酶（MMPs）对于细胞外基质的降解/重塑至关重要，并参与着床期间螺旋动脉的形成和子宫内膜的侵袭。已知MMP9启动子中的一个功能性单核苷酸多态性（SNP），即-1562C/T，以等位基因特异性方式影响基因表达。本研究评估了MMP9基因SNP -1562C/T的母体基因型与不孕女性体外受精和胚胎移植（IVF-ET）结局之间的关联。这项病例对照研究包括不孕患者（n = 123）和有一个健康孩子的女性作为对照（n = 147）。通过PCR/RFLP对SNP-1562C/T进行基因分型。患者和对照之间的等位基因和基因型分布没有显著差异（P>0.05）。123例病例中MMP9基因型频率为C/C = 73.17%，C/T = 24.40%，T/T = 2.43%；C和T等位基因频率分别为85.36%和14.63%。147例对照中MMP9基因型频率为C/C = 73.46%，C/T = 20.40%，T/T = 6.12%；C和T等位基因频率分别为83.67%和16.32%。总之，本研究结果表明，在该人群中MMP9基因的SNP -1562C/T可能与IVF-ET结局无关。
