Moeinfar Sadegheh, Mashayekhi Farhad, Bahadori Mohammad Hadi, Faraji Roya, Salehi Zivar
Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
Cellular and Molecular Research Center, Guilan University of Medical Sciences, Rasht, Iran.
J Reprod Infertil. 2023 Jan-Mar;24(1):11-17. doi: 10.18502/jri.v24i1.11904.
Vascular endothelial growth factor receptors (VEGFR) play an important role in embryo implantation. The aim of the present study was to examine the association of VEGFR1 circulating level and gene polymorphism with in vitro fertilization and embryo transfer (IVF-ET) outcome.
In this case-control study, 120 women who had unsuccessful IVF (IVF) history and 120 women who had successful IVF outcome (IVF) as controls were included. Genomic DNA was extracted from blood samples. Genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The serum levels of soluble VEGFR1 (sVEGFR1) were measured by ELISA. ANOVA test was used for statistical analysis.
The frequency of T and C alleles in IVF individuals were 87.5%, 12.5% and among IVF were 75.5%, 24.5%, respectively (p=0.0006). The minor allele (C) was associated with an increased risk of IVF failure based on results from co-dominant (OR=3.86, 95%CI 1.19-12.47), dominant (OR=2.32, 95%CI 1.31-4.10), recessive (OR=3.22, 95%CI 1.00-10.29), and allele models (OR=2.28, 95%CI 1.40-3.69). We also showed that there is a significant decrease in serum sVEGFR1 levels in IVF as compared to IVF (p=0.006) groups. Moreover, TT genotype is significantly associated with increased serum sVEGFR1 concentration in IVF group (TT, CT, and CC serum levels were 106.55±11.04, 94.33±10.75, and 83.33±9.13 , and in IVF group were 156.11±18.08, 120.66±16.51, and 84.66±20.31 , respectively).
The results of this study indicate that VEGFR1 polymorphism and sVEGFR1 circulating levels are associated with IVF-ET outcome. Moreover, CC genotype is associated with decreased sVEGFR-1 serum concentration and IVF-ET failure.
血管内皮生长因子受体（VEGFR）在胚胎着床过程中发挥重要作用。本研究旨在探讨VEGFR1循环水平及基因多态性与体外受精-胚胎移植（IVF-ET）结局之间的关联。
在这项病例对照研究中，纳入了120例有体外受精（IVF）失败史的女性和120例体外受精成功的女性作为对照。从血样中提取基因组DNA。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）进行基因分型。通过酶联免疫吸附测定（ELISA）检测可溶性VEGFR1（sVEGFR1）的血清水平。采用方差分析进行统计学分析。
IVF个体中T和C等位基因的频率分别为87.5%、12.5%，IVF个体中分别为75.5%、24.5%（p=0.0006）。基于共显性（OR=3.86，95%可信区间1.19-12.47）、显性（OR=2.32，95%可信区间1.31-4.10）、隐性（OR=3.22，95%可信区间1.00-10.29）及等位基因模型（OR=2.28，95%可信区间1.40-3.69）的结果，次要等位基因（C）与IVF失败风险增加相关。我们还发现，与IVF组相比，IVF组血清sVEGFR1水平显著降低（p=0.006）。此外，IVF组中TT基因型与血清sVEGFR1浓度升高显著相关（TT、CT和CC的血清水平分别为106.55±11.04、94.33±10.75和83.33±9.13，IVF组分别为156.11±18.08、120.66±16.51和84.66±20.31）。
本研究结果表明，VEGFR1多态性及sVEGFR1循环水平与IVF-ET结局相关。此外，CC基因型与sVEGFR-1血清浓度降低及IVF-ET失败相关。
