Wang Hui, Lin Xian-Feng, Wang Li-Ren, Lin Yi-Qian, Wang Jiang-Tao, Liu Wen-Yue, Zhu Gui-Qi, Braddock Martin, Zhong Ming, Zheng Ming-Hua
Department of Neurosurgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
Expert Rev Neurother. 2015 May;15(5):493-500. doi: 10.1586/14737175.2015.1030735. Epub 2015 Mar 27.
Decellularization methodologies have been successfully used in a variety of tissue engineering and regenerative technologies and methods of decellularization have been developed for target tissues and organs of interest. The technology to promote regeneration and functional recovery in the CNS, including brain and spinal cord, has, however, made slow progress mainly because the intrinsic regenerative potential of the CNS is regarded as low. To date, currently available therapies have been unable to provide significant functional recovery and successful therapies, which could provide functional restoration to the injured brain and spinal cord are controversial. In this review, the authors provide a critical analysis, comparing the advantages and limitations of the major decellularization methods and considering the effects of these methods upon the biologic scaffold material. The authors also review studies that supplement decellularized grafts with exogenous factors, such as stem cells and growth factors, to both promote and enhance regeneration through decellularized allografts.
脱细胞技术已成功应用于多种组织工程和再生技术，并且已经针对感兴趣的靶组织和器官开发了脱细胞方法。然而，促进中枢神经系统（包括脑和脊髓）再生和功能恢复的技术进展缓慢，主要是因为中枢神经系统的内在再生潜力被认为较低。迄今为止，现有的治疗方法无法实现显著的功能恢复，而能够为受伤的脑和脊髓提供功能恢复的成功疗法仍存在争议。在本综述中，作者进行了批判性分析，比较了主要脱细胞方法的优缺点，并考虑了这些方法对生物支架材料的影响。作者还回顾了通过用干细胞和生长因子等外源性因子补充脱细胞移植物，以促进并增强脱细胞同种异体移植物再生的研究。
