Gilbert Thomas W, Sellaro Tiffany L, Badylak Stephen F
McGowan Institute for Regenerative Medicine, University of Pittsburgh, 100 Technology Drive, Suite 200, Pittsburgh, PA 15219, USA.
Biomaterials. 2006 Jul;27(19):3675-83. doi: 10.1016/j.biomaterials.2006.02.014. Epub 2006 Mar 7.
Decellularized tissues and organs have been successfully used in a variety of tissue engineering/regenerative medicine applications, and the decellularization methods used vary as widely as the tissues and organs of interest. The efficiency of cell removal from a tissue is dependent on the origin of the tissue and the specific physical, chemical, and enzymatic methods that are used. Each of these treatments affect the biochemical composition, tissue ultrastructure, and mechanical behavior of the remaining extracellular matrix (ECM) scaffold, which in turn, affect the host response to the material. Herein, the most commonly used decellularization methods are described, and consideration give to the effects of these methods upon the biologic scaffold material.
脱细胞组织和器官已成功应用于各种组织工程/再生医学领域，所使用的脱细胞方法因感兴趣的组织和器官的不同而有很大差异。从组织中去除细胞的效率取决于组织的来源以及所使用的特定物理、化学和酶促方法。这些处理方法中的每一种都会影响剩余细胞外基质（ECM）支架的生化组成、组织超微结构和力学行为，进而影响宿主对该材料的反应。本文描述了最常用的脱细胞方法，并考虑了这些方法对生物支架材料的影响。
