水解条件对海藻酸盐及其衍生物中天冬氨酸到古洛糖醛酸比率检测的影响。
Impact of hydrolysis conditions on the detection of mannuronic to guluronic acid ratio in alginate and its derivatives.
机构信息
Jiangsu Key Laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou 215021, Jiangsu, China.
Department of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Department of Pharmacy, Qingdao Central Hospital, Qingdao 266042, Shandong, China.
出版信息
Carbohydr Polym. 2015 May 20;122:180-8. doi: 10.1016/j.carbpol.2015.01.008. Epub 2015 Jan 12.
Alginate is a linear and acidic polysaccharide, composed of (1 → 4) linked β-D-mannuronic acid (ManA) and α-L-guluronic acid (GulA). The ratio of ManA to GulA (M/G) is one of the most important factors for the application of alginate and its derivatives in various areas. In this work, a robust and accurate method was developed to analyze M/G using high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The impact of hydrolysis conditions on the release patterns of ManA and GulA from alginate and its derivatives was investigated. The release patterns of ManA and GulA need to be considered separately to obtain an accurate M/G. Several hydrolysis conditions were established that released ManA and GulA completely and maintained these saccharide residues intact. The proper M/G of alginates from different sources and its derivatives could then be calculated by integration of the corresponding ManA and GulA peaks.
藻酸盐是一种线性酸性多糖，由（1→4）键合的β-D-甘露糖醛酸（ManA）和α-L-古洛糖醛酸（GulA）组成。ManA 与 GulA 的比例（M/G）是藻酸盐及其衍生物在各个领域应用的最重要因素之一。在这项工作中，开发了一种使用高效阴离子交换色谱-脉冲安培检测（HPAEC-PAD）分析 M/G 的强大而准确的方法。研究了水解条件对藻酸盐及其衍生物中 ManA 和 GulA 释放模式的影响。需要分别考虑 ManA 和 GulA 的释放模式，以获得准确的 M/G。建立了几种水解条件，可完全释放 ManA 和 GulA 并保持这些糖残基完整。然后，通过整合相应的 ManA 和 GulA 峰，可以计算出不同来源的藻酸盐及其衍生物的适当 M/G。
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