Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980, Curitiba, Brazil.
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980, Curitiba, Brazil.
Carbohydr Polym. 2015 May 20;122:276-81. doi: 10.1016/j.carbpol.2014.12.085. Epub 2015 Jan 14.
Primary cell wall polysaccharides from aqueous extract of buriti fruit pulp (Mauritia flexuosa, an exotic tropical palm) were isolated and characterized. After freeze-thaw and α-amylase treatments, extracted polysaccharides were purified by sequential ultrafiltration through membranes. Two homogeneous fractions were obtained, SBW-100R and SBW-30R (Mw of 126 kDa and 20 kDa, respectively). Monosaccharide composition, methylation and (13)C NMR analysis showed that fraction SBW-100R contained a (1 → 5)-linked arabinan, branched at O-3 and O-2 positions, linked to a type I rhamnogalacturonan. Low amounts of these polymers were also present in fraction SBW-30R according to (13)C NMR analysis and monosaccharide composition. However, a high methyl esterified homogalacturonan (HG) was present in higher proportions. These results reinforce previous findings present in literature data which indicate that pectic polysaccharides are found in high amounts in primary cell walls of palms, which are commelinid monocotyledons.
从巴西栗（Mauritia flexuosa，一种外来热带棕榈）果肉的水提物中分离和表征了初生细胞壁多糖。经过冻融和α-淀粉酶处理后，提取的多糖通过顺序超滤进行纯化，通过膜过滤。得到了两种均一的级分，SBW-100R 和 SBW-30R（Mw 分别为 126 kDa 和 20 kDa）。单糖组成、甲基化和（13）C NMR 分析表明，级分 SBW-100R 含有（1 → 5）连接的阿拉伯聚糖，在 O-3 和 O-2 位置支化，与 I 型鼠李半乳糖醛酸聚糖连接。根据（13）C NMR 分析和单糖组成，SBW-30R 中也存在少量这些聚合物。然而，存在大量高甲酯化的同质半乳糖醛酸聚糖（HG）。这些结果加强了文献数据中先前的发现，表明果胶多糖在 commelinid 单子叶植物的初生细胞壁中含量很高。
