Lewin M B, Wong A, Deveney C W, Sankaran H
FEBS Lett. 1985 May 20;184(2):259-62. doi: 10.1016/0014-5793(85)80618-9.
The Ca2+ ionophore, A23187, stimulated amylase secretion from isolated rat pancreatic acini in a dose-dependent manner with a maximal effect at 6 microM. Acetaldehyde, a metabolite of ethanol, caused a reduction in the magnitude of ionophore-stimulated secretion with no evidence of competitive inhibition. Furthermore, 6 microM ionophore-stimulated amylase secretion was dose-dependently inhibited by acetaldehyde. This inhibitory effect of acetaldehyde, however, was reversible on washing and reincubating acetaldehyde-treated acini. These results suggest that acetaldehyde reversibly inhibits intracellular components mediating stimulated secretion and this inhibition requires a continuous chemical interaction between acetaldehyde and intracellular component(s) regulating stimulated enzyme secretion.
钙离子载体A23187以剂量依赖的方式刺激分离的大鼠胰腺腺泡分泌淀粉酶，在6微摩尔时达到最大效应。乙醇的代谢产物乙醛可使离子载体刺激的分泌量减少，且无竞争性抑制的证据。此外，6微摩尔离子载体刺激的淀粉酶分泌受到乙醛的剂量依赖性抑制。然而，乙醛的这种抑制作用在冲洗并重新孵育经乙醛处理的腺泡后是可逆的。这些结果表明，乙醛可逆地抑制介导刺激分泌的细胞内成分，且这种抑制需要乙醛与调节刺激酶分泌的细胞内成分之间持续的化学相互作用。
