乙醛对分离的大鼠胰腺腺泡中胆囊收缩素诱导的淀粉酶分泌的不可逆抑制作用。

Irreversible inhibition by acetaldehyde of cholecystokinin-induced amylase secretion from isolated rat pancreatic acini.

作者信息

Sankaran H, Lewin M B, Wong A, Deveney C W, Wendland M F, Leimgruber R M, Geokas M C

出版信息

Biochem Pharmacol. 1985 Aug 15;34(16):2859-63. doi: 10.1016/0006-2952(85)90007-3.

Abstract

Acetaldehyde inhibited both amylase secretion induced by maximal concentrations (300 pM) of cholecystokinin octapeptide and the binding of radioiodinated cholecystokinin to receptors on isolated rat pancreatic acini. This inhibition was concentration dependent (10 mM to 1 M for amylase secretion and 100 mM to 1 M for binding). However, a correlation between the two inhibitory effects could not be obtained. Furthermore, the inhibitory effects were not reversible. Acetaldehyde did not alter the basal amylase secretion between 6 and 45 mM concentrations. However, 60, 100 and 300 mM acetaldehyde significantly decreased basal amylase secretion; no significant change in amylase secretion was observed at 600 mM and 1 M. Higher concentrations of acetaldehyde produced a 2- to 10-fold increase in basal amylase secretion. 51Cr release from prelabeled acini revealed no significant cell membrane damage between 10 and 600 mM acetaldehyde. These data suggest that acetaldehyde inhibition of cholecystokinin-induced amylase secretion is intracellularly mediated.

摘要

乙醛可抑制由最大浓度（300 pM）的八肽胆囊收缩素诱导的淀粉酶分泌，以及放射性碘化胆囊收缩素与离体大鼠胰腺腺泡上受体的结合。这种抑制作用呈浓度依赖性（淀粉酶分泌为10 mM至1 M，结合为100 mM至1 M）。然而，未能得出这两种抑制作用之间的相关性。此外，抑制作用是不可逆的。在6至45 mM浓度范围内，乙醛不会改变基础淀粉酶分泌。然而，60、100和300 mM的乙醛可显著降低基础淀粉酶分泌；在600 mM和1 M时，未观察到淀粉酶分泌有显著变化。更高浓度的乙醛可使基础淀粉酶分泌增加2至10倍。从预先标记的腺泡中释放的51Cr显示，在10至600 mM乙醛之间，细胞膜无明显损伤。这些数据表明，乙醛对胆囊收缩素诱导的淀粉酶分泌的抑制作用是由细胞内介导的。