氮掺杂石墨烯量子点的固有类过氧化物酶催化活性及其在H2O2和葡萄糖比色检测中的应用。
Intrinsic peroxidase-like catalytic activity of nitrogen-doped graphene quantum dots and their application in the colorimetric detection of H2O2 and glucose.
作者信息
Lin Liping, Song Xinhong, Chen Yiying, Rong Mingcong, Zhao Tingting, Wang Yiru, Jiang Yaqi, Chen Xi
机构信息
Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
Xiamen Huaxia College, Xiamen 361024, China.
出版信息
Anal Chim Acta. 2015 Apr 15;869:89-95. doi: 10.1016/j.aca.2015.02.024. Epub 2015 Feb 13.
In this paper, the highly intrinsic peroxidase-like catalytic activity of nitrogen-doped graphene quantum dots (N-GQDs) is revealed. This activity was greatly dependent on pH, temperature and H2O2 concentration. The experimental results showed that the stable N-GQDs could be used for the detection of H2O2 and glucose over a wide range of pH and temperature, offering a simple, highly selective and sensitive approach for their colorimetric sensing. The linearity between the analyte concentration and absorption ranged from 20 to 1170 μM for H2O2 and 25 to 375 μM for glucose with a detection limit of 5.3 μM for H2O2 and 16 μM for glucose. This assay was also successfully applied to the detection of glucose concentrations in diluted serum and fruit juice samples.
本文揭示了氮掺杂石墨烯量子点（N-GQDs）高度固有的类过氧化物酶催化活性。该活性在很大程度上取决于pH值、温度和H2O2浓度。实验结果表明，稳定的N-GQDs可用于在较宽的pH值和温度范围内检测H2O2和葡萄糖，为其比色传感提供了一种简单、高选择性和灵敏的方法。对于H2O2，分析物浓度与吸光度之间的线性范围为20至1170μM，对于葡萄糖为25至375μM，H2O2的检测限为5.3μM，葡萄糖的检测限为16μM。该测定法也成功应用于稀释血清和果汁样品中葡萄糖浓度的检测。
Zotero 插件