Roark M, Mahoney P A, Graham M L, Lengyel J A
Dev Biol. 1985 Jun;109(2):476-88. doi: 10.1016/0012-1606(85)90473-7.
We have isolated, by molecular cloning, genes expressed differentially at the blastoderm stage of Drosophila melanogaster. Two of the blastoderm-differential genes are reexpressed at later stages, and map to single chromosomal loci 95C and 99E. The sequence at 99E is that encoding the myosin light chain 2. Two other blastoderm-differential sequences are members of multigene families (one of which is B104, or roo) and map to multiple dispersed chromosomal loci. A gastrula-differential sequence was found which maps to 71A. Most significantly, we have identified three genes encoding transcripts expressed uniquely at the blastoderm stage; these map to single chromosomal loci: 25D3, 75C, and 99D4-8. At least some of the blastoderm-differential and blastoderm-specific loci appear to be distinct from loci involved in embryonic pattern formation that have been identified in recent genetic "saturation" screens. The procedure of identifying genes specific to the blastoderm stage may thus allow the identification of genes, not previously identified by classical genetic techniques, that are involved in important embryonic processes.
我们通过分子克隆分离出了在黑腹果蝇胚盘阶段差异表达的基因。其中两个胚盘差异基因在后期阶段重新表达，并定位于单个染色体位点95C和99E。99E处的序列是编码肌球蛋白轻链2的序列。另外两个胚盘差异序列是多基因家族的成员（其中一个是B104，即roo），并定位于多个分散的染色体位点。发现了一个原肠胚差异序列，定位于71A。最显著的是，我们鉴定出了三个编码仅在胚盘阶段表达的转录本的基因；它们定位于单个染色体位点：25D3、75C和99D4-8。至少一些胚盘差异位点和胚盘特异性位点似乎与最近的遗传“饱和”筛选中鉴定出的参与胚胎模式形成的位点不同。因此，鉴定胚盘阶段特异性基因的过程可能会使我们鉴定出一些以前通过经典遗传技术未鉴定出的、参与重要胚胎过程的基因。
