Wiedmann K H, Trejdosiewicz L K, Goodall A H, Thomas H C
Gut. 1985 May;26(5):510-7. doi: 10.1136/gut.26.5.510.
Eight monoclonal antibodies have been raised to antigenic determinants within the liver specific lipoprotein complex. Five of these determinants were species and the others non-species specific. No liver specific determinants were identified. Liver specific lipoprotein antigens recognised by the eight monoclonal antibodies were located either on the hepatocyte membrane and/or along the sinusoidal lining wall or in the cytoplasm of liver parenchymal cells. All antibodies but one reacted with the cell membrane of viable human hepatocytes. The molecular weights of four liver specific lipoprotein-determinants were determined by immunoprecipitation. These ranged from 22 000 to 164 000 daltons.
已针对肝脏特异性脂蛋白复合物中的抗原决定簇制备了8种单克隆抗体。其中5种决定簇具有种属特异性，其他则不具有种属特异性。未鉴定出肝脏特异性决定簇。这8种单克隆抗体识别的肝脏特异性脂蛋白抗原位于肝细胞膜上和/或沿窦状隙衬壁，或位于肝实质细胞的细胞质中。除一种抗体外，所有抗体均与活的人肝细胞的细胞膜发生反应。通过免疫沉淀法测定了4种肝脏特异性脂蛋白决定簇的分子量。这些分子量范围为22000至164000道尔顿。
