Wiedmann K H, Bartholemew T C, Brown D J, Thomas H C
Hepatology. 1984 Mar-Apr;4(2):199-204. doi: 10.1002/hep.1840040206.
In this study, we describe a radioimmunoassay to detect liver membrane binding antibodies. The assay was designed to exclude binding of aggregated IgG or immune complexes to Fc gamma receptors of hepatocytes. When this assay was applied to sera from 142 patients, antibodies were found in highest titer in patients with autoimmune chronic active hepatitis, rarely in patients with hepatitis B virus-induced chronic active liver disease, and in 32% of patients with primary biliary cirrhosis. IgM antibodies were found in 100% of patients with acute Type A but not B or non-A, non-B hepatitis. IgA class antibodies were found in the sera of 57% of patients with alcohol-induced hepatitis. All patient groups showing significant titers of liver membrane antibodies display the lesion of piecemeal necrosis except those with alcohol-induced hepatitis. Further studies are needed to determine whether this antibody is the cause of the lesion.
在本研究中，我们描述了一种用于检测肝细胞膜结合抗体的放射免疫测定法。该测定法旨在排除聚集的IgG或免疫复合物与肝细胞Fcγ受体的结合。当将此测定法应用于142例患者的血清时，在自身免疫性慢性活动性肝炎患者中发现抗体滴度最高，在乙型肝炎病毒引起的慢性活动性肝病患者中很少见，而在32%的原发性胆汁性肝硬化患者中存在。在100%的急性甲型肝炎患者中发现IgM抗体，但在乙型或非甲非乙型肝炎患者中未发现。在57%的酒精性肝炎患者血清中发现了IgA类抗体。除酒精性肝炎患者外，所有显示肝细胞膜抗体滴度显著的患者组均表现为碎片状坏死病变。需要进一步研究以确定这种抗体是否是病变的原因。
