Dikow A, Meschkov T, Pfleiderer G
Folia Haematol Int Mag Klin Morphol Blutforsch. 1985;112(1):46-54.
For the ultracytochemical identification of alkaline phosphatase in lymphocytes gained from the peripheral blood of healthy individuals a sensitive method is described which allows the low enzyme activity of these cells to be determined. This was possible because the authors succeeded in stabilizing lead ions in the alkaline medium by forming a complex directly between tris-(hydroxymethyl) aminomethan and lead (II) citrate. AP localized ultrachemically in lymphocytes in particular formations similar to phosphasomes of neutrophilic granulocytes. In those lymphocytes stimulated by lipopolysaccharides a high enzyme activity could be observed and, in addition to phosphasomes, the product of response can also be found in canal-like structures of the endoplasmatic reticulum. These findings contribute to clarify the ultrastructural localization of alkaline phosphatase in lymphocytes and may be regarded as an aid in discovering the importance of the enzyme in the biology of lymphocytes or in its activation, respectively.
为了对从健康个体外周血中获取的淋巴细胞中的碱性磷酸酶进行超微细胞化学鉴定，本文描述了一种灵敏的方法，该方法可用于测定这些细胞中的低酶活性。之所以能够做到这一点，是因为作者通过三（羟甲基）氨基甲烷与柠檬酸铅（II）直接形成络合物，成功地在碱性介质中稳定了铅离子。超微化学定位显示，淋巴细胞中的碱性磷酸酶存在于特定结构中，类似于嗜中性粒细胞的磷酸小体。在那些受到脂多糖刺激的淋巴细胞中，可以观察到高酶活性，除了磷酸小体之外，在内质网的管状结构中也能发现反应产物。这些发现有助于阐明碱性磷酸酶在淋巴细胞中的超微结构定位，并且分别可以被视为有助于发现该酶在淋巴细胞生物学或其激活过程中的重要性的辅助手段。
