Jing Huai-Zhi, Qiu Feng, Chen Shi-Zhi, Su Lin, Qu Can
Department of Pharmacy, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2015 Mar;35(3):437-41.
To investigate the expression of tripartite-motif protein 25 (TRIM25) and pyruvate kinase M2 (PKM2) protein in non-small cell lung cancer (NSCLC) and explore their role in the occurrence and progression of NSCLC.
The expressions of TRIM25 and PKM2 protein were detected in 60 NSCLC specimens and 20 adjacent normal lung tissue (>5 cm from the lesions) with immunofluorescence histochemical method and in 10 fresh specimens of NSCLC with Western blotting. The results were analyzed in relation with the clinicopathological features of the patients.
The positivity rates of TRIM25 expression was 45% in the 60 lung carcinoma specimens, significantly higher than that in the 20 normal lung tissues (10%, P=0.005). TRIM25 protein was expressed in 28.6% of lung adenocarcinoma tissues and in 59.4% of squamous carcinoma tissues (P=0.017). TRIM25 protein expression was positively correlated with the TNM stages and lymph node metastasis of NSCLC (P<0.05). The expressions of PKM2 protein in 60 cases of lung carcinoma was 73.3%,while in 20 cases of normal lung tissues the expressions was 30%(P=0.001). The positivity rates of PKM2 expression differed significantly between lung adenocarcinoma and squamous carcinoma (57.1% vs 87.5%, P=0.008). An inverse correlation was noted between TRIM25 and PKM2 expressions (P=0.026).
TRIM25 and PKM2 protein may participate in the occurrence and progression of NSCLC, and their expressions are inversely correlated.
探讨三联基序蛋白25（TRIM25）和丙酮酸激酶M2（PKM2）蛋白在非小细胞肺癌（NSCLC）中的表达情况，并探究它们在NSCLC发生发展中的作用。
采用免疫荧光组织化学方法检测60例NSCLC标本及20例癌旁正常肺组织（距病灶>5 cm）中TRIM25和PKM2蛋白的表达，并采用蛋白质印迹法检测10例新鲜NSCLC标本中二者的表达。分析结果与患者临床病理特征的关系。
60例肺癌标本中TRIM25表达阳性率为45%，显著高于20例正常肺组织中的阳性率（10%，P=0.005）。TRIM25蛋白在肺腺癌组织中的表达率为28.6%，在肺鳞癌组织中的表达率为59.4%（P=0.017）。TRIM25蛋白表达与NSCLC的TNM分期及淋巴结转移呈正相关（P<0.05）。60例肺癌组织中PKM2蛋白的表达率为73.3%，20例正常肺组织中的表达率为30%（P=0.001）。PKM2在肺腺癌和肺鳞癌中的阳性表达率差异有统计学意义（57.1%对87.5%，P=0.008）。TRIM25与PKM2的表达呈负相关（P=0.026）。
TRIM25和PKM2蛋白可能参与NSCLC的发生发展，且二者表达呈负相关。
