[丙酮酸激酶M2型和低氧诱导因子-1α在喉鳞状细胞癌中的表达及其临床意义]

[The expression of PKM2 and HIF-1α in laryngeal squamous cell carcinoma and its clinical indication].

作者信息

Liu Xiaoxiao, Shan Chunguang, Xu Ou, Wang Jingmiao, Zhang Haizhong

出版信息

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2015 Jul;29(13):1152-5.

Abstract

OBJECTIVE

The purpose of this study was to investigate the expression of PKM2 and HIF-1α in laryngeal squamous cell carcinoma and to analyze their correlation in laryngeal squamous cell carcinoma.

METHOD

Total 37 laryngeal carcinoma samples and para-carcinoma tissues were taken from the patients who accepted operation treatment in the Second Hospital of HeBei Medical University from 06/2013 to 06/2014. The protein expression levels of PKM2 and HIF-1α were detected with SP immunohistochemical methods. The data were analyzed by the SPSS 13.0 statistical software.

RESULT

The positive expression of PKM2 in laryngeal carcinoma tissues and adjacent tissues were 62.16% and 13.15%. The difference was statistically significant (P < 0.01). The positive expression of HIF-1α in laryngeal carcinoma tissues and adjacent tissues were 64.86% and 21.62%. The difference was statistically significant (P < 0.01). The positive expression of PKM2 and HIF-1α in well differentiated laryngeal squamous cell carcinoma were both 47.83%, while in medium and poorly differentiated laryngeal squamous cell carcinoma were 85.71% and 92.86% respectively. The difference was statistically significant (P < 0.05). The positive expression of PKM2 and HIF-1α in patients with lymph metastasis were 90.00% and 100.00% respectively, 51.85% in those without lymph metastasis, the difference was statistically significant (P < 0.05). The rate of HIF-1α positive expression in I-II stage was 53.85%, 90.91% in III-IV stage. The difference between the two groups was statistically significant (P < 0.05). The expression of PKM2 and HIF-1α had no relationship with the age and smoking (P > 0.05). The expression of PKM2 was positively related with HIF-1α in laryngeal squamous cell carcinoma (P < 0.01).

CONCLUSION

The expression of PKM2 and HIF-1α are related with the proliferation, invasion and metastasis of laryngeal squamous cell carcinoma. It provides a certain theoretical basis for laryngeal cancer diagnosis and screening to measure the expression of PKM2 and HIF-1α as biological indicators.

摘要

目的

本研究旨在探讨丙酮酸激酶M2（PKM2）和缺氧诱导因子-1α（HIF-1α）在喉鳞状细胞癌中的表达情况，并分析它们在喉鳞状细胞癌中的相关性。

方法

选取2013年6月至2014年6月在河北医科大学第二医院接受手术治疗的患者的37份喉癌组织及癌旁组织。采用SP免疫组化法检测PKM2和HIF-1α的蛋白表达水平。数据用SPSS 13.0统计软件进行分析。

结果

PKM2在喉癌组织和癌旁组织中的阳性表达率分别为62.16%和13.15%，差异有统计学意义（P<0.01）。HIF-1α在喉癌组织和癌旁组织中的阳性表达率分别为64.86%和21.62%，差异有统计学意义（P<0.01）。高分化喉鳞状细胞癌中PKM2和HIF-1α的阳性表达率均为47.83%，中低分化喉鳞状细胞癌中分别为85.71%和92.86%，差异有统计学意义（P<0.05）。有淋巴结转移患者中PKM2和HIF-1α的阳性表达率分别为90.00%和100.00%，无淋巴结转移患者中为分别为51.85%，差异有统计学意义（P<0.05）。HIF-1α在Ⅰ-Ⅱ期的阳性表达率为53.85%，Ⅲ-Ⅳ期为90.91%，两组差异有统计学意义（P<0.05）。PKM2和HIF-1α的表达与年龄及吸烟无关（P>0.05）。喉鳞状细胞癌中PKM2的表达与HIF-1α呈正相关（P<0.01）。