Marchenko Mikhail, Thomson Andrew, Ellis Terri N, Knuckley Bryan, Causey Corey P
Department of Chemistry, University of North Florida, 1 UNF Dr., Jacksonville, FL 32224, USA.
Department of Biology, University of North Florida, 1 UNF Dr., Jacksonville, FL 32224, USA.
Bioorg Med Chem. 2015 May 1;23(9):2159-67. doi: 10.1016/j.bmc.2015.03.013. Epub 2015 Mar 9.
Agmatine deiminases (AgDs) catalyze the hydrolytic conversion of agmatine (decarboxylated arginine) to N-carbamoylputrescine with concomitant release of ammonia. These enzymes, which are encoded by some pathogenic bacterial species, confer a competitive survival advantage by virtue of energy production and acid tolerance through agmatine catabolism. Herein we report the development of a clickable activity-based protein profiling (ABPP) probe that targets the AgD encoded by Streptococcus mutans with high selectivity and sensitivity.
胍丁胺脱亚氨酶（AgDs）催化胍丁胺（脱羧精氨酸）水解转化为N-氨甲酰腐胺，并伴随氨的释放。这些由一些致病细菌物种编码的酶，通过胍丁胺分解代谢产生能量和耐受酸性环境，从而赋予细菌竞争生存优势。在此，我们报道了一种基于活性的可点击蛋白质谱分析（ABPP）探针的开发，该探针能以高选择性和敏感性靶向变形链球菌编码的AgD。
