Wu Ai-Min, Ni Wen-Fei, Huang Zhe-Yu, Li Qing-Long, Wu Jian-Bo, Xu Hua-Zi, Yin Li-Hui
Laboratory of Internal Medicine, The First Affiliated Hospital of Wenzhou Medical University, 2# Fuxue Road, Wenzhou 325027, People's Republic of China; The Department of Spinal Surgery, Second Affiliated Hospital of Wenzhou Medical University, Zhejiang Spinal Research Center, 109# XueYuan Western Road, Wenzhou, Zhejiang 325027, People's Republic of China.
The Department of Spinal Surgery, Second Affiliated Hospital of Wenzhou Medical University, Zhejiang Spinal Research Center, 109# XueYuan Western Road, Wenzhou, Zhejiang 325027, People's Republic of China.
J Neurol Sci. 2015 Apr 15;351(1-2):160-167. doi: 10.1016/j.jns.2015.03.011. Epub 2015 Mar 12.
Many studies have reported micro RNAs involved in the differentiation of bone marrow mesenchymal stem cells (BMSCs) into neural cells; however, the roles of long non-coding RNAs (lncRNAs) in the differentiation of BMSCs into neural cells remain poorly understood. We used microarray assays to compare the lncRNA and messenger RNA (mRNA) expression profiles in BMSCs and neural-induced BMSCs. We found a total of 24 lncRNAs and 738 mRNAs that were upregulated and 32 lncRNAs and 682 mRNAs that were downregulated in samples induced for 3h; 27 lncRNAs and 864 mRNAs that were upregulated and 37 lncRNAs and 968 mRNAs that were downregulated in 6h samples; and 23 lncRNAs and 1159 mRNAs that were upregulated or downregulated in both the 3h and 6h samples. For 23 differentially lncRNAs and 83 differentially mRNAs, 256 matched lncRNA-mRNA pairs were found. GO (Gene ontology) analysis showed that these lncRNAs were associated with biological processes, cellular components, and molecular functions. Twenty-five pathways were identified by pathway analysis. Then, RT-qPCR validation of the differentially expressed H19, Esco2, Pcdhb18, and RGD1560277 genes confirmed the microarray data. Our study revealed the expression patterns of lncRNAs in the differentiation of BMSCs into neural cells, and many lncRNAs were differentially expressed in induced BMSCs, suggesting that they may play key roles in processes of differentiation. Our findings may promote the use of BMSCs to treat neurodegenerative diseases and trauma.
许多研究报道了参与骨髓间充质干细胞（BMSC）向神经细胞分化的微小RNA；然而，长链非编码RNA（lncRNA）在BMSC向神经细胞分化中的作用仍知之甚少。我们使用微阵列分析来比较BMSC和神经诱导BMSC中的lncRNA和信使RNA（mRNA）表达谱。我们发现在诱导3小时的样本中，共有24种lncRNA和738种mRNA上调，32种lncRNA和682种mRNA下调；在诱导6小时的样本中，27种lncRNA和864种mRNA上调，37种lncRNA和968种mRNA下调；在3小时和6小时的样本中，均有23种lncRNA和1159种mRNA上调或下调。对于23种差异lncRNA和83种差异mRNA，发现了256对匹配的lncRNA-mRNA对。基因本体论（GO）分析表明，这些lncRNA与生物学过程、细胞成分和分子功能相关。通过通路分析确定了25条通路。然后，对差异表达的H19、Esco2、Pcdhb18和RGD1560277基因进行逆转录-定量聚合酶链反应（RT-qPCR）验证，证实了微阵列数据。我们的研究揭示了lncRNA在BMSC向神经细胞分化中的表达模式，并且许多lncRNA在诱导的BMSC中差异表达，表明它们可能在分化过程中起关键作用。我们的发现可能会促进BMSC在治疗神经退行性疾病和创伤方面的应用。
