天然和变性DNA在葡聚糖凝胶G200上的固定化。
Immobilization of native and denatured DNA on Sephadex G200.
作者信息
Mykoniatis M G
出版信息
J Biochem Biophys Methods. 1985 Mar;10(5-6):321-8. doi: 10.1016/0165-022x(85)90067-3.
An efficient method for the immobilization of DNA on Sephadex G200 in the presence of water soluble carbodiimide is described and investigated in this paper. An increase in the extent of binding was observed when the incubation temperature of the DNA-Sephadex mixture was changed. It was found that native DNA immobilized to Sephadex with higher efficiency than denatured DNA. However, the stability of native DNA-Sephadex complex was about the same as that of denatured DNA-Sephadex. The size of DNA released by DNA-Sephadex after incubation of a suspension of the complex was the same as that of the DNA used for immobilization. The binding mechanism of DNA to Sephadex is discussed.
本文描述并研究了一种在水溶性碳二亚胺存在下将DNA固定在葡聚糖凝胶G200上的有效方法。当改变DNA - 葡聚糖凝胶混合物的孵育温度时，观察到结合程度增加。发现天然DNA比变性DNA更有效地固定在葡聚糖凝胶上。然而，天然DNA - 葡聚糖凝胶复合物的稳定性与变性DNA - 葡聚糖凝胶的稳定性大致相同。复合物悬浮液孵育后，DNA - 葡聚糖凝胶释放的DNA大小与用于固定的DNA大小相同。本文还讨论了DNA与葡聚糖凝胶的结合机制。
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