van der Ploeg M, Duijndam W A
Histochemistry. 1986;84(4-6):283-300. doi: 10.1007/BF00482953.
An overview is given of the preparation and use of model systems for cytochemistry, dealing with quantitative as well as qualitative aspects. Descriptions are given of the various possibilities to prepare cytochemical matrix models, ranging from macroscopic and microscopic films, to models with more cell-like dimensions as agarose beads, artificial cells and erythrocyte ghosts. Such models allow the study of a large variety of cytochemical processes. Their potentialities are demonstrated in a number of specific applications, comprising: the study of the influence of fixation on cellular processes, reaction specificity and reaction kinetics, quality of reagents and biochemical calibration in cytochemical staining; factors influencing localization of the specific endproduct in enzyme cytochemistry; immunocytochemistry and hybridocytochemistry.
本文概述了细胞化学模型系统的制备和应用，涉及定量和定性方面。介绍了制备细胞化学基质模型的各种可能性，从宏观和微观薄膜到具有更类似细胞尺寸的模型，如琼脂糖珠、人工细胞和红细胞血影。此类模型可用于研究多种细胞化学过程。在一些具体应用中展示了它们的潜力，包括：固定对细胞过程、反应特异性和反应动力学的影响研究；细胞化学染色中试剂质量和生化校准；酶细胞化学中影响特定终产物定位的因素；免疫细胞化学和杂交细胞化学。
