Schrader W P, West C A
J Histochem Cytochem. 1985 Jun;33(6):508-14. doi: 10.1177/33.6.2582028.
Adenosine deaminase complexing proteins have been localized in four exocrine glands of the rabbit by immunoperoxidase staining employing affinity-purified goat anti-rabbit complexing protein immunoglobulin as the primary antibody. In pancreatic acinar cells and in serous cells of Brunner glands (duodenal glands), staining was concentrated in granular appearing deposits between the nucleus and cell apex. Bile canaliculi, components of the exocrine liver, were also positive for complexing protein. In submaxillary glands, staining was localized in serous demilunes and striated ducts. In each instance staining was blocked by preincubating the primary antibody with complexing protein purified from rabbit kidney.
采用亲和纯化的山羊抗兔复合蛋白免疫球蛋白作为一抗，通过免疫过氧化物酶染色法，已将腺苷脱氨酶复合蛋白定位在兔的四种外分泌腺中。在胰腺腺泡细胞和布伦纳腺（十二指肠腺）的浆液性细胞中，染色集中在细胞核与细胞顶端之间呈颗粒状的沉积物中。外分泌性肝脏的组成部分胆小管，对复合蛋白也呈阳性反应。在颌下腺中，染色定位于浆液半月体和纹状管。在每种情况下，通过将一抗与从兔肾中纯化的复合蛋白预孵育，可阻断染色。
