Chechik B E, Baumal R, Sengupta S
Histochem J. 1983 Apr;15(4):373-87. doi: 10.1007/BF01002970.
Rabbit antibody to calf adenosine deaminase (ADA) was used to localize this enzyme in tissues of the young rat and calf by the immunoperoxidase method. The distribution patterns of ADA in most tissues were similar for both species. Within the thymus gland, the enzyme was strongly expressed predominantly in cortical lymphocytes. In the spleen and lymph nodes, most lymphocytes of T-cell areas stained weakly for ADA, whereas only a small number of ADA-positive cells were found in B-cell areas. Clumps of strongly ADA-positive mononuclear blastoid and plasma cells were observed in the medullary regions of lymph nodes, around peri-arteriolar lymphocyte sheaths and in the red pulp of the spleen, and in the lamina propria of the intestine. Double immunofluorescence staining studies in the rat showed that some of these blastoid cells contained both ADA and immunoglobulins and appeared to be plasmablasts. Strong staining for ADA was also found, in both the rat and calf, in as yet unidentified mononuclear blastoid cells in the interstitium of non-lymphoid organs (kidney, heart, lung), in endothelial cells of some arterioles and capillaries, and in Kupffer cells of the liver. In addition, ADA was strongly expressed in calf bile canaliculi. These studies define areas in rat and calf tissues which contain ADA-positive cells and provide a model system for investigations of the relationship between ADA and the function and development of these cells.
用兔抗小牛腺苷脱氨酶（ADA）抗体，通过免疫过氧化物酶法在幼鼠和小牛组织中定位该酶。两种物种大多数组织中ADA的分布模式相似。在胸腺内，该酶主要在皮质淋巴细胞中强烈表达。在脾脏和淋巴结中，T细胞区的大多数淋巴细胞ADA染色较弱，而在B细胞区仅发现少量ADA阳性细胞。在淋巴结髓质区、动脉周围淋巴细胞鞘周围、脾脏红髓以及肠固有层中观察到大量强ADA阳性的单核母细胞样细胞和浆细胞。大鼠的双重免疫荧光染色研究表明，这些母细胞样细胞中的一些同时含有ADA和免疫球蛋白，似乎是成浆细胞。在大鼠和小牛的非淋巴器官（肾脏、心脏、肺）间质中尚未鉴定的单核母细胞样细胞、一些小动脉和毛细血管的内皮细胞以及肝脏的库普弗细胞中也发现了ADA的强染色。此外，ADA在小牛胆小管中强烈表达。这些研究确定了大鼠和小牛组织中含有ADA阳性细胞的区域，并为研究ADA与这些细胞的功能和发育之间的关系提供了一个模型系统。
