Sorensen C M, Pierce C W
J Immunol. 1986 Sep 1;137(5):1455-61.
In vitro activation of naive spleen cells from C57BL/10 mice with GAT and the monoclonal GAT-TsF1, 372B3.5, followed by fusion with BW5147 resulted in generation of a hybridoma that fails to produce GAT-TsF constitutively, but upon reexposure to GAT and 372B3.5 is induced to secrete GAT-TsF2. The induction is GAT specific and requires de novo RNA, protein synthesis, and DNA synthesis. Although both GAT and 372B3.5 are required for induction, they may be added sequentially, provided the GAT is added first. The GAT-TsF produced by the induced cell is antigen specific and composed of two polypeptide chains: one capable of binding antigen, the other bearing determinants encoded by the I-J region of the MHC. The utility of this inducible GAT-TsF2 cell line for molecular biology and other studies is discussed.
用谷氨酸-丙氨酸-酪氨酸（GAT）和单克隆GAT- TsF1（372B3.5）体外激活C57BL/10小鼠的脾细胞，然后与BW5147细胞融合，产生了一种杂交瘤，该杂交瘤不能组成性地产生GAT- TsF，但在再次暴露于GAT和372B3.5后被诱导分泌GAT- TsF2。这种诱导是GAT特异性的，需要从头进行RNA、蛋白质合成和DNA合成。虽然诱导需要GAT和372B3.5两者，但它们可以依次添加，前提是先添加GAT。诱导细胞产生的GAT- TsF具有抗原特异性，由两条多肽链组成：一条能够结合抗原，另一条带有由MHC的I-J区域编码的决定簇。讨论了这种可诱导的GAT- TsF2细胞系在分子生物学和其他研究中的用途。
