Liu Jian, Zhang Lili, Fu Chuanyun, Wang Yunshan, Sun Shanhui
School of Control Science and Engineering, Shandong University, Jinan, 250061, China.
School of Mechanical and Automotive Engineering, Qilu University of Technology, Jinan, 250353, China.
Luminescence. 2015 Dec;30(8):1297-302. doi: 10.1002/bio.2896. Epub 2015 Mar 25.
Highly sensitive detection of hepatitis C virus (HCV) in serum is a key method for diagnosing and classifying the extent of HCV infection. In this study, a p-phenol derivative, 4-(1,2,4-triazol-1-yl)phenol (4-TRP), was employed as an efficient enhancer of the luminol-hydrogen peroxide (H2O2)-horseradish peroxidase (HRP) chemiluminescence (CL) system for detection of HCV. Compared with a traditional enhancer, 4-TRP strongly enhanced CL intensity with the effect of prolonging and stabilizing light emission. The developed CL system was applied to detecting HCV core antigen (HCV-cAg) using a sandwich structure inside microwells. Our experimental results showed that there was good linear relationship between CL intensity and HCV-cAg concentration in the 0.6-3.6 pg/mL range (R = 0.99). The intra- and inter-assay coefficients of variation were 4.5-5.8% and 5.0-7.3%, respectively. In addition, sensitive determination of HCV-cAg in serum samples using the luminol-H2O2-HRP-4-TRP CL system was also feasible in clinical settings.
血清中丙型肝炎病毒（HCV）的高灵敏度检测是诊断和分类HCV感染程度的关键方法。在本研究中，对苯酚衍生物4-(1,2,4-三唑-1-基)苯酚（4-TRP）被用作鲁米诺-过氧化氢（H2O2）-辣根过氧化物酶（HRP）化学发光（CL）体系检测HCV的有效增强剂。与传统增强剂相比，4-TRP强烈增强了CL强度，并具有延长和稳定发光的效果。所开发的CL体系用于在微孔板内采用夹心结构检测HCV核心抗原（HCV-cAg）。我们的实验结果表明，在0.6-3.6 pg/mL范围内，CL强度与HCV-cAg浓度之间存在良好的线性关系（R = 0.99）。批内和批间变异系数分别为4.5-5.8%和5.0-7.3%。此外，在临床环境中使用鲁米诺-H2O2-HRP-4-TRP CL体系灵敏测定血清样本中的HCV-cAg也是可行的。
