Chen Cuimei, Ding Ping
School of Public Health, Xiangnan University, Chenzhou 423000, China.
Xiangya School of Public Health, Central South University, Changsha 410078, China.
Molecules. 2024 Dec 4;29(23):5722. doi: 10.3390/molecules29235722.
To explore the optimization method and application of Au-NP-enhanced luminol--HO luminescence system in TORCH (TOX, RV, CMV, HSVI, and HSVII) detection.
4.5 × 10 mmol/L gold nano solution was prepared with chloroauric acid as the reducing agent and trisodium citrate as the stabilizer. After curing for 3 days, Au NPs participate in the luminal-HO luminescence system to detect TORCH antibodies and establish the cut off value. SPSS 18.0 software was used to analyze the TORCH antibodies detected by the nano-gold-enhanced luminol luminescence method and TORCH kit. Additionally, its detection performance is studied.
The results of a paired -test for the absorbance values of samples with and without gold nanoparticles showed that there were statistically significant differences ( < 0.001) between the two methods in the detection of TOX, RV, CMV, HSVI, and HSVII. The luminescence values with the addition of gold nanoparticles were significantly higher than those without gold nanoparticles. Using the Au NP-luminol-HO chemiluminescence method, 127 serum samples were tested for TORCH antibodies. The sensitivities were 84.6%, 83.3%, 90.9%, 85.7%, and 84.6%, while the specificities were 94.7%, 96.5%, 96.6%, 97.3%, and 95.6%, respectively. The sensitivity and specificity of the chemiluminescence method enhanced by gold nanoparticles are significantly improved compared to the chemiluminescence method without enhancers.
Au NPs participate in the luminal-HO luminescent system. The absorbance, sensitivity, and specificity of TORCH antibodies show that Au NPs can enhance the luminol-HO luminescent system. Au NP-luminol-HO luminescence system has broad application prospects in the detection of eugenics.
探讨金纳米颗粒（Au-NP）增强鲁米诺-H₂O₂发光体系在TORCH（弓形虫、风疹病毒、巨细胞病毒、单纯疱疹病毒Ⅰ型和单纯疱疹病毒Ⅱ型）检测中的优化方法及应用。
以氯金酸为还原剂、柠檬酸钠为稳定剂制备4.5×10⁻³ mmol/L金纳米溶液。固化3天后，金纳米颗粒（Au NPs）参与鲁米诺-H₂O₂发光体系检测TORCH抗体并确定临界值。使用SPSS 18.0软件分析纳米金增强鲁米诺发光法和TORCH试剂盒检测的TORCH抗体，并研究其检测性能。
对添加和未添加金纳米颗粒的样品吸光度值进行配对检验，结果显示两种方法在检测弓形虫、风疹病毒、巨细胞病毒、单纯疱疹病毒Ⅰ型和单纯疱疹病毒Ⅱ型时存在统计学显著差异（P<0.001）。添加金纳米颗粒后的发光值明显高于未添加金纳米颗粒的发光值。采用Au NP-鲁米诺-H₂O₂化学发光法检测127份血清样本的TORCH抗体，敏感性分别为84.6%、83.3%、90.9%、85.7%和84.6%，特异性分别为94.7%、96.5%、96.6%、97.3%和95.6%。与未加增强剂的化学发光法相比，金纳米颗粒增强的化学发光法的敏感性和特异性显著提高。
金纳米颗粒（Au NPs）参与鲁米诺-H₂O₂发光体系。TORCH抗体的吸光度、敏感性和特异性表明Au NPs可增强鲁米诺-H₂O₂发光体系。Au NP-鲁米诺-H₂O₂发光体系在优生学检测中具有广阔的应用前景。
