Bocian Renata, Kazmierska Paulina, Kłos-Wojtczak Paulina, Kowalczyk Tomasz, Konopacki Jan
Department of Neurobiology, Faculty of Biology and Environmental Protection, University of Lodz, Poland.
Hippocampus. 2015 Nov;25(11):1393-406. doi: 10.1002/hipo.22459. Epub 2015 Apr 15.
Previous in vivo data suggested that orexin neuropeptides (ORX(A) and ORX(B) ) synthetized in hypothalamic neurons were involved in the mechanism of generation of the hippocampal formation theta rhythm. Surprisingly, this suggestion has never been directly proved by experiments using intraseptal or intrahippocampal administration of orexins. In this study, involving the use of in vitro hippocampal formation slices and in vivo model of anesthetized rat, we provide the first convergent electropharmacological evidence that in the presence of both ORX(A) and ORX(B) the hippocampal formation neuronal network is capable of producing oscillations in the theta band. This effect of orexin peptides was antagonized by selective blockers of orexin receptors (OX1 R and OX2 R), SB 334867 and TCS OX2 29, respectively. These results provide evidence for a novel, orexinergic mechanism responsible for the production of theta rhythm in the hippocampal formation neuronal network.
先前的体内实验数据表明，在下丘脑神经元中合成的食欲素神经肽（ORX(A)和ORX(B)）参与了海马结构θ节律的产生机制。令人惊讶的是，这一推测从未通过在隔区内或海马区内注射食欲素的实验得到直接证实。在本研究中，我们使用体外海马结构切片和麻醉大鼠的体内模型，首次提供了一致的电药理学证据，表明在同时存在ORX(A)和ORX(B)的情况下，海马结构神经元网络能够产生θ频段的振荡。食欲素肽的这种作用分别被食欲素受体（OX1 R和OX2 R）的选择性阻断剂SB 334867和TCS OX2 29所拮抗。这些结果为一种新的、负责海马结构神经元网络中θ节律产生的食欲素能机制提供了证据。
