用于检测鲑科鱼类细菌性肾病的诊断程序的比较敏感性。

Comparative sensitivities of diagnostic procedures used to detect bacterial kidney disease in salmonid fishes.

作者信息

Cipriano R C, Starliper C E, Schachte J H

出版信息

J Wildl Dis. 1985 Apr;21(2):144-8. doi: 10.7589/0090-3558-21.2.144.

DOI:10.7589/0090-3558-21.2.144

Abstract

Kidney and spleen homogenates from each of 60 coho salmon (Oncorhynchus kisutch) and steelhead trout (Salmo gairdneri) were examined for detection of Renibacterium salmoninarum. The proportions of positives differed widely with the detection procedures used: in coho salmon, 5% were positive by the Gram-stain procedure, 10% by the direct fluorescent antibody test, 48% by bacteriological isolation, 65% by staphylococcal coagglutination, and 73% by counterimmunoelectrophoresis; in steelhead trout, 3% were positive by Gram-stain, 8.3% by fluorescent antibody, 17% by bacteriological isolation, and 67% by counterimmunoelectrophoresis. Renibacterium salmoninarum was not detected in either coho salmon or steelhead trout by immunodiffusion analysis.

摘要

对60条银大麻哈鱼（Oncorhynchus kisutch）和虹鳟（Salmo gairdneri）的肾脏和脾脏匀浆进行检测，以确定是否存在鲑肾杆菌（Renibacterium salmoninarum）。使用不同检测方法时阳性比例差异很大：在银大麻哈鱼中，革兰氏染色法检测阳性率为5%，直接荧光抗体检测为10%，细菌分离法为48%，葡萄球菌协同凝集法为65%，对流免疫电泳法为73%；在虹鳟中，革兰氏染色法阳性率为3%，荧光抗体法为8.3%，细菌分离法为17%，对流免疫电泳法为67%。免疫扩散分析在银大麻哈鱼和虹鳟中均未检测到鲑肾杆菌。