通过毛细管电泳-高分辨率质谱法对天然产物提取物中已知核碱基和核苷化合物进行去重复分析。

Dereplication of known nucleobase and nucleoside compounds in natural product extracts by capillary electrophoresis-high resolution mass spectrometry.

作者信息

Chen Junhui, Shi Qian, Wang Yanlong, Li Zhaoyong, Wang Shuai

机构信息

Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China.

College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China.

出版信息

Molecules. 2015 Mar 26;20(4):5423-37. doi: 10.3390/molecules20045423.

DOI:10.3390/molecules20045423

PMID:25822081

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6272742/

Abstract

Nucleobase and nucleoside compounds exist widely in various organisms. An often occurring problem in the discovery of new bioactive compounds from natural products is reisolation of known nucleobase and nucleoside compounds. To resolve this problem, a capillary electrophoresis-high resolution mass spectrometry (CE-HR-MS) method providing both rapid separation and accurate mass full-scan MS data was developed for the first time to screen and dereplicate known nucleobase and nucleoside compounds in crude extracts of natural products. Instrumental parameters were optimized to obtain optimum conditions for CE separation and electrospray ionization-time-of-flight mass spectrometry (ESI-TOF/MS) detection. The proposed method was verified to be precise, reproducible, and sensitive. Using this method, known nucleobase and nucleoside compounds in different marine medicinal organisms including Syngnathus acus Linnaeus; Hippocampus japonicus Kaup and Anthopleura lanthogrammica Berkly were successfully observed and identified. This work demonstrates that CE-HR-MS combined with an accurate mass database may be used as a powerful tool for dereplicating known nucleobase and nucleoside compounds in different types of natural products. Rapid dereplication of known nucleobase and nucleoside compounds allows researchers to focus on other leads with greater potential to yield new substances.

摘要

核碱基和核苷化合物广泛存在于各种生物体中。从天然产物中发现新的生物活性化合物时经常出现的一个问题是已知核碱基和核苷化合物的重新分离。为了解决这个问题，首次开发了一种毛细管电泳-高分辨率质谱（CE-HR-MS）方法，该方法既能提供快速分离又能提供准确的全扫描质谱数据，用于筛选和去除天然产物粗提物中已知的核碱基和核苷化合物。对仪器参数进行了优化，以获得毛细管电泳分离和电喷雾电离-飞行时间质谱（ESI-TOF/MS）检测的最佳条件。所提出的方法被验证具有精确性、可重复性和灵敏性。使用该方法，成功观察并鉴定了不同海洋药用生物（包括尖海龙、日本海马和红斑海葵）中已知的核碱基和核苷化合物。这项工作表明，CE-HR-MS结合精确质量数据库可作为一种强大的工具，用于去除不同类型天然产物中已知的核碱基和核苷化合物。快速去除已知的核碱基和核苷化合物使研究人员能够专注于其他更有潜力产生新物质的线索。