Qingdao Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, First Institute Oceanography of SOA, Qingdao, P. R. China.
J Sep Sci. 2010 Mar;33(4-5):672-7. doi: 10.1002/jssc.200900544.
In this paper, a method based on HPLC coupled with ESI-TOF/MS and on-line to a stable free radical scavenging detection system has been developed to rapidly screen and identify the major antioxidants in the water extract of Hippocampus japonicus Kaup, a kind of marine medicinal organism. The developed on-line stable free radical scavenging detection method of water extract from H. japonicus Kaup revealed the presence of three radical scavenging compounds, which were simultaneously identified as hypoxanthine, phenylalanine and tryptophane by high-resolution TOF-MS. To the best of our knowledge, it is original to demonstrate the rapid and successful use of HPLC-ESI-TOF/MS and on-line 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) diammonium salt free radical scavenging assay to directly and simultaneously screen and identify the antioxidants in the water extracts of H. japonicus Kaup without any purification. The present method provides a useful improvement on the reducing of workload and a powerful tool for rapid screening and identification of free radical scavenging compounds in complex natural products.
本文建立了一种基于 HPLC 与 ESI-TOF/MS 联用，并在线连接稳定自由基清除检测系统的方法，用于快速筛选和鉴定海洋药用生物海马水提物中的主要抗氧化剂。所建立的在线稳定自由基清除检测方法可用于检测海马水提物，从中发现了 3 种自由基清除化合物，通过高分辨 TOF-MS 同时鉴定为次黄嘌呤、苯丙氨酸和色氨酸。据我们所知，这是首次证明 HPLC-ESI-TOF/MS 与在线 2,2'-联氮-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐自由基清除测定法可直接、同时筛选和鉴定未经任何纯化的海马水提物中的抗氧化剂，这是一种快速且成功的应用。本方法为减少工作量提供了有益的改进，也是快速筛选和鉴定复杂天然产物中自由基清除化合物的有力工具。
