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海胆胚胎分离细胞核中U1 RNA的合成：U1 RNA在RNA的第一个核苷酸处起始合成。

Synthesis of U1 RNA in isolated nuclei from sea urchin embryos: U1 RNA is initiated at the first nucleotide of the RNA.

作者信息

Morris G F, Marzluff W F

出版信息

Mol Cell Biol. 1985 May;5(5):1143-50. doi: 10.1128/mcb.5.5.1143-1150.1985.

DOI:10.1128/mcb.5.5.1143-1150.1985
PMID:2582239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC366833/
Abstract

Nuclei from sea urchin blastula embryos synthesize a variety of small RNAs, one of which has identical mobility with sea urchin U1 RNA. This RNA is synthesized by RNA polymerase II and, in a hybridization-selection experiment, was selected by the cloned sea urchin U1 gene. The U1 RNA was initiated with ATP, but not GTP, in isolated nuclei with beta-S- and gamma-S-ribonucleotide triphosphates as substrates. The U1 RNA containing thiophosphate at the 5' end was not capped but accumulated as an uncapped transcript from which the thiophosphate could be removed with calf intestinal phosphatase.

摘要

海胆囊胚胚胎的细胞核合成多种小RNA，其中一种与海胆U1 RNA具有相同的迁移率。这种RNA由RNA聚合酶II合成，在杂交选择实验中，被克隆的海胆U1基因所选择。在以β-S-和γ-S-核糖核苷酸三磷酸为底物的分离细胞核中，U1 RNA以ATP而非GTP起始。5'端含有硫代磷酸的U1 RNA没有被加帽，而是作为未加帽的转录本积累，硫代磷酸可以用小牛肠磷酸酶去除。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e022/366833/6205a2299aef/molcellb00101-0250-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e022/366833/bddb2b315590/molcellb00101-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e022/366833/27105941e9ce/molcellb00101-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e022/366833/615bb5f65df9/molcellb00101-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e022/366833/c3fd43f8db65/molcellb00101-0250-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e022/366833/6205a2299aef/molcellb00101-0250-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e022/366833/bddb2b315590/molcellb00101-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e022/366833/27105941e9ce/molcellb00101-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e022/366833/615bb5f65df9/molcellb00101-0249-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e022/366833/c3fd43f8db65/molcellb00101-0250-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e022/366833/6205a2299aef/molcellb00101-0250-b.jpg

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1
Synthesis of U1 RNA in isolated nuclei from sea urchin embryos: U1 RNA is initiated at the first nucleotide of the RNA.海胆胚胎分离细胞核中U1 RNA的合成：U1 RNA在RNA的第一个核苷酸处起始合成。
Mol Cell Biol. 1985 May;5(5):1143-50. doi: 10.1128/mcb.5.5.1143-1150.1985.
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A conserved region in the sea urchin U1 snRNA promoter interacts with a developmentally regulated factor.海胆U1小核RNA启动子中的一个保守区域与一种发育调控因子相互作用。
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本文引用的文献

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Identification of the RNA region transferred from a representative primer, beta-globin mRNA, to influenza mRNA during in vitro transcription.在体外转录过程中，鉴定从代表性引物β-珠蛋白mRNA转移至流感病毒mRNA的RNA区域。
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Structure and expression of a second sea urchin U1 RNA gene repeat.第二种海胆U1 RNA基因重复序列的结构与表达
Nucleic Acids Res. 1986 Dec 22;14(24):9977-88. doi: 10.1093/nar/14.24.9977.
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Sea urchin maternal and embryonic U1 RNAs are spatially segregated in early embryos.海胆母体和胚胎的U1核糖核酸在早期胚胎中呈空间隔离状态。
J Cell Biol. 1987 May;104(5):1133-42. doi: 10.1083/jcb.104.5.1133.
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Synthesis of U1 RNA in a DNA-dependent system from sea urchin embryos.海胆胚胎DNA依赖系统中U1 RNA的合成。
Proc Natl Acad Sci U S A. 1986 Jun;83(11):3674-8. doi: 10.1073/pnas.83.11.3674.
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Synthesis of U1 RNA in isolated mouse cell nuclei: initiation and 3'-end formation.小鼠离体细胞核中U1 RNA的合成：起始与3'端形成
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Proc Natl Acad Sci U S A. 1989 Apr;86(8):2572-6. doi: 10.1073/pnas.86.8.2572.
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Cell. 1982 Jul;29(3):877-86. doi: 10.1016/0092-8674(82)90449-4.
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The organization and expression of histone gene families.组蛋白基因家族的组织与表达
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