Morris G F, Marzluff W F
Biochemistry. 1983 Feb 1;22(3):645-53. doi: 10.1021/bi00272a019.
Isolated nuclei from sea urchin embryos synthesize RNA at a rate comparable to other animal cell nuclei. All three RNA polymerases are active as judged by alpha-amanitin sensitivity and hybridization to specific cloned DNAs. Extracts were prepared from sea urchin eggs and embryos by extraction with 0.35 M KCl. None of the crude extracts had a large effect on total RNA synthesis. However, extracts from sea urchin eggs inhibited RNA polymerase III activity in nuclei from blastula and gastrula embryos. There was no effect on the synthesis of ribosomal RNA by RNA polymerase I or on the synthesis of two RNA polymerase II products, histone mRNA and the sea urchin analogue of U1 RNA. The inhibitor is present in two different species of sea urchin and has been 50-fold purified by diethylaminoethylcellulose and hydroxylapatite chromatography. The inhibitor is not present in extracts prepared from sea urchin blastula embryos.
从海胆胚胎中分离出的细胞核合成RNA的速率与其他动物细胞核相当。根据α-鹅膏蕈碱敏感性以及与特定克隆DNA的杂交判断，所有三种RNA聚合酶均具有活性。通过用0.35M KCl提取，从海胆卵和胚胎中制备提取物。粗提取物均未对总RNA合成产生显著影响。然而，海胆卵提取物抑制囊胚和原肠胚胚胎细胞核中的RNA聚合酶III活性。对RNA聚合酶I合成核糖体RNA或对两种RNA聚合酶II产物（组蛋白mRNA和U1 RNA的海胆类似物）的合成没有影响。该抑制剂存在于两种不同的海胆物种中，并已通过二乙氨基乙基纤维素和羟基磷灰石色谱法进行了50倍纯化。该抑制剂不存在于从海胆囊胚胚胎制备的提取物中。
