Xavier A M, Rai K, Hegde A M, Shetty S
Department of Pediatric dentistry, Amrita School of Dentistry.
Dept of Pedodontics and Preventive Dentistry, Bangalore Institute of Dental Sciences.
J Clin Pediatr Dent. 2015 Winter;39(2):161-7. doi: 10.17796/jcpd.39.2.g52v661835527526.
This study aimed to compare the in vitro mineral loss and surface microhardness (SMH) changes in human enamel specimens following supplementation of acidic carbonated beverages with low iron concentrations than when treated without.
180 enamel blocks each from primary and permanent teeth were prepared and equally subdivided (n=10) for their respective treatments in Group 1 (Coca Cola and Sprite without iron supplementation) and Group 2 (beverages supplemented with 2/5 mmol/L FeSO₄.7H₂O). Following initial SMH estimation, the blocks were subjected to 3 treatment cycles of 5/20 minute incubation periods, equally interspaced by a 5-min treatment in artificial saliva. The calcium and phosphate released after each cycle were analyzed spectrophotometrically and the final SMH was recorded. The results were tested using student's T test, One-way ANOVA and Kruskal Walli's test (p<0.05).
Two and five mmol/L FeSO₄.7H₂O supplementation produced a highly significant SMH change and calcium and phosphate reduction than when treated without (p<.0005). Both the enamel specimens showed similar patterns of mineral loss and SMH reduction, with pronounced effects in the twenty minute incubation cycles.
Our results suggest that 2 mmol/L FeSO₄.7H₂O supplementation to acidic beverages is beneficial in reducing mineral loss and preserving surface microhardness of human enamel.
本研究旨在比较补充低铁浓度酸性碳酸饮料的人牙釉质标本与未补充时的体外矿物质损失和表面显微硬度（SMH）变化。
制备180个分别来自乳牙和恒牙的牙釉质块，并将其平均分成若干组（n = 10），分别在第1组（未补充铁的可口可乐和雪碧）和第2组（补充2/5 mmol/L FeSO₄·7H₂O的饮料）中进行各自的处理。在初步估计SMH后，将这些牙釉质块进行3个处理周期，每个周期包括5/20分钟的孵育期，并在人工唾液中进行5分钟的处理，各周期之间均匀间隔。对每个周期后释放的钙和磷进行分光光度分析，并记录最终的SMH。使用学生t检验、单因素方差分析和Kruskal Walli检验对结果进行检验（p<0.05）。
与未补充时相比，补充2 mmol/L和5 mmol/L FeSO₄·7H₂O导致SMH发生极显著变化，钙和磷减少（p<0.0005）。两种牙釉质标本均表现出相似的矿物质损失和SMH降低模式，在20分钟孵育周期中影响显著。
我们的结果表明，向酸性饮料中补充2 mmol/L FeSO₄·7H₂O有利于减少矿物质损失并保持人牙釉质的表面显微硬度。
