Yang Yong, Li Dianbo, Yang Yang, Jiang Gening
Department of Thoracic Surgery, Shanghai Pulmonary Hospital Affiliated Tongji University, Shanghai 200433, P.R. China.
Department of Thoracic Surgery, Linyi Tumor Hospital, Linyi, Shandong 276001, P.R. China.
Mol Med Rep. 2015 Jul;12(1):945-52. doi: 10.3892/mmr.2015.3557. Epub 2015 Mar 27.
Esophageal squamous cell cancer (ESCC) is an aggressive type of cancer with poor prognosis and leading to decreased quality of life. The identification of patients at increased risk of esophageal squamous cell cancer may improve current understanding of the role of micro (mi)RNA in tumorigenesis, since the miRNA pattern of these patients may be associated with tumorigenesis. In the present study, the miRNA and mRNA expression profiles of ESCC tissue samples and adjacent normal control tissue samples were obtained from two dependent GEO series. Bioinformatics analyses, including the use of the Gene Oncology and Kyoto Encyclopedia of Genes and Genomes databases, were used to identify genes and pathways, which were specifically associated with miRNA-associated ESCC oncology. A total of 17 miRNAs and 1,670 probes were differentially expressed in the two groups, and the differentially expressed miRNA and target interactions were analyzed. The mRNA of miRNA target genes were found to be involve 49 GO terms and 14 pathways. Of the genes differentially expressed between the two groups, miRNA-181a, miRNA-202, miRNA-155, FNDC3B, BNC2 and MBD2 were the most significantly altered and may be important in the regulatory network. In the present study, a novel pattern of differential miRNA-target expression was constructed, which with further investigation, may provide novel targets for diagnosing and understanding the mechanism of ESCC.
食管鳞状细胞癌（ESCC）是一种侵袭性癌症，预后较差，会导致生活质量下降。识别食管鳞状细胞癌风险增加的患者，可能会增进目前对微小（mi）RNA在肿瘤发生中作用的理解，因为这些患者的miRNA模式可能与肿瘤发生有关。在本研究中，ESCC组织样本和相邻正常对照组织样本的miRNA和mRNA表达谱取自两个相关的基因表达综合数据库（GEO）系列。生物信息学分析，包括使用基因本体论和京都基因与基因组百科全书数据库，用于识别与miRNA相关的ESCC肿瘤学特异性相关的基因和通路。两组共有17种miRNA和1670个探针差异表达，并对差异表达的miRNA及其靶标相互作用进行了分析。发现miRNA靶基因的mRNA涉及49个基因本体（GO）术语和14条通路。在两组之间差异表达的基因中，miRNA-181a、miRNA-202、miRNA-155、富含纤连蛋白结构域3B（FNDC3B）、BNC2和甲基化结合结构域2（MBD2）变化最为显著，可能在调控网络中起重要作用。在本研究中，构建了一种新的差异miRNA-靶标表达模式，经进一步研究，可能为ESCC的诊断和了解其机制提供新的靶点。
