DNA链交换疏水催化的证据。
Evidence for hydrophobic catalysis of DNA strand exchange.
作者信息
Feng B, Westerlund F, Nordén B
机构信息
Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Kemivägen 10, SE-412 96 Gothenburg, Sweden.
出版信息
Chem Commun (Camb). 2015 Apr 30;51(34):7390-2. doi: 10.1039/c5cc01515d.
PMID:25823973
Abstract
The catalytic role of hydrophobic co-solutes in DNA strand exchange is demonstrated by FRET kinetics. Two mechanisms that contribute to this are base stacking destabilisation and nucleation-promoted DNA strand invasion. We propose that hydrophobic catalysis is involved in the strand-exchange activity of recombination enzymes.
摘要
荧光共振能量转移动力学证明了疏水性共溶质在DNA链交换中的催化作用。促成此作用的两种机制是碱基堆积失稳和成核促进的DNA链侵入。我们提出，疏水催化参与了重组酶的链交换活性。
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