Hara H, Moriki T, Hiroi M, Yamane T
Pathol Res Pract. 1985 Mar;179(4-5):499-503.
The purpose of this study was to examine effects of aphidicolin and alpha-amanitin on visualization of acridine orange (AO) binding to DNA in rat astrocytoma C6 cells and to discuss briefly the significance of AO chromatin interaction products. Aphidicolin inhibited DNA synthesis but percentage of AO positive cells was approximately 60% of that of the untreated control cells. alpha-Amanitin caused a slight inhibition of RNA synthesis and 3H-uridine incorporation in the treated cells was about 64% of that of the untreated cells, whereas a distinct decrease of the number of AO positive cell nuclei was observed. The results suggest that activity of RNA polymerase II and mRNA synthesis is mainly concerned in visualization of AO chromatin interaction products.
本研究的目的是检测阿非迪霉素和α-鹅膏蕈碱对吖啶橙（AO）与大鼠星形细胞瘤C6细胞中DNA结合的可视化效果，并简要讨论AO染色质相互作用产物的意义。阿非迪霉素抑制DNA合成，但AO阳性细胞的百分比约为未处理对照细胞的60%。α-鹅膏蕈碱对RNA合成有轻微抑制作用，处理细胞中3H-尿苷掺入量约为未处理细胞的64%，而观察到AO阳性细胞核数量明显减少。结果表明，RNA聚合酶II的活性和mRNA合成主要与AO染色质相互作用产物的可视化有关。
