Chessa M G, Zoncheddu A, Accomando R, Crippa Franceschi T
Boll Soc Ital Biol Sper. 1983 Aug 30;59(8):1142-8.
The ciliated protozoan Colpoda cucullus has been cultivated at 27 degrees C with gentle shaking in a baked lettuce infusion supplemented with Klebsiella suspensions. Under these conditions cells had a mean generation time of about 7 hours and could attain densities up to 20,000/ml and 45,000/ml in the log and stationary phase of growth, respectively. Nuclear preparations obtained from exponentially growing cells by the gum arabic-octanol method showed a satisfactory degree of purity and integrity. They consisted primarily of the large macronuclei attached to which the small micronuclei were sometimes visible. Upon incubation at 27 degrees C in conventional reaction mixtures nuclear preparations actively incorporated 3H-UTP and 3H-dTTP into acid-insoluble material. alpha-amanitin caused a 50% inhibition of RNA synthesis whereas aphidicolin did not affect at all DNA synthesis.
纤毛原生动物尾草履虫在27摄氏度下，于添加了克雷伯氏菌悬液的烘焙生菜浸出液中轻轻摇晃培养。在这些条件下，细胞的平均世代时间约为7小时，在对数生长期和稳定期的密度分别可达20,000/ml和45,000/ml。通过阿拉伯胶 - 辛醇法从指数生长的细胞中获得的细胞核制剂显示出令人满意的纯度和完整性。它们主要由大的巨核组成，有时可见附着在其上的小的微核。在传统反应混合物中于27摄氏度孵育时，细胞核制剂能将3H-UTP和3H-dTTP积极掺入酸不溶性物质中。α-鹅膏蕈碱对RNA合成有50%的抑制作用，而阿非迪霉素对DNA合成完全没有影响。
