Hashimura T, Tubbs R R, Connelly R, Caulfield M J, Trindade C S, McMahon J T, Galetti T P, Edinger M, Sandberg A A, Dal Cin P
Department of Urology, Cleveland Clinic Foundation, Ohio 44195-5123.
Cancer Res. 1989 Dec 15;49(24 Pt 1):7064-71.
Two human renal carcinoma cell lines have been established from the same patient. One cell line (CCF-RC1) was obtained from the primary tumor and the second (CCF-RC2) was established from cells of the renal vein effluent of the perfused tumorous kidney. Although they were established from the same patient, the cell lines differed in certain biological properties. They have been passaged up to 50 times in vitro for about two years. Each has an epithelial morphology and exhibits mutilayering. Cell cycle time of CCF-RC1 and CCF-RC2 was 34 and 36 h, respectively. They exhibited anchorage independent growth, and the plating efficiency of CCF-RC2 in soft agar was higher than that of CCF-RC1. Both lines induced tumors in nude mice at the site of s.c. injection closely resembling the original tumor in histological examination. Electron microscopic features of both tumors in nude mice were consistent with epithelial origin. Doubling time of CCF-RC1 and CCF-RC2 in nude mice was 11 and 12 days, respectively. CCF-RC1 and CCF-RC2 have hypotetraploid karyotype and modal numbers of 83 and 73, presenting two and three marker chromosomes, respectively. Immunocytology with commercial monoclonal antibodies against renal carcinoma (URO-3) and cytokeratin (Mac 6) showed positive reactions with both lines, suggesting that these cell lines derived from renal epithelium. A murine monoclonal antibody (2E11) was generated against CCF-RC2 by the hybridoma technique; 2E11 reacted with CCF-RC2, but not with CCF-RC1. These cell lines may provide a useful model for the study of tumor heterogeneity and its relationship to metastasis.
从同一患者体内建立了两个人肾癌细胞系。一个细胞系（CCF-RC1）取自原发性肿瘤，另一个（CCF-RC2）则由灌注的患肾肾静脉流出液中的细胞建立而成。尽管它们来自同一患者，但这两个细胞系在某些生物学特性上存在差异。它们已在体外传代约两年，传代次数达50次。每个细胞系都具有上皮细胞形态并呈现多层生长。CCF-RC1和CCF-RC2的细胞周期时间分别为34小时和36小时。它们表现出不依赖贴壁生长，并且CCF-RC2在软琼脂中的接种效率高于CCF-RC1。将这两个细胞系皮下注射到裸鼠体内后均能诱导肿瘤形成，组织学检查显示这些肿瘤与原始肿瘤极为相似。裸鼠体内这两种肿瘤的电子显微镜特征均与上皮起源一致。CCF-RC1和CCF-RC2在裸鼠体内的倍增时间分别为11天和12天。CCF-RC1和CCF-RC2具有亚四倍体核型，众数分别为83和73，分别呈现两条和三条标记染色体。使用抗肾癌（URO-3）和细胞角蛋白（Mac 6）的商业单克隆抗体进行免疫细胞化学检测，结果显示这两个细胞系均呈阳性反应，表明这些细胞系源自肾上皮细胞。通过杂交瘤技术制备了一种针对CCF-RC2的鼠单克隆抗体（2E11）；2E11与CCF-RC2反应，但不与CCF-RC1反应。这些细胞系可能为研究肿瘤异质性及其与转移的关系提供有用的模型。
