Buszello H, Ackermann R
Department of Urology, Heinrich-Heine-University Düsseldorf, Germany.
Anticancer Res. 1994 Jan-Feb;14(1A):173-6.
Two new human cell lines (DNT-11 and DNT-63) derived from renal cell carcinoma metastases were established and characterized. They have been passaged up to 50 times in vitro for more than 4 years. Each line has an epithelial morphology. Cell cycle time of DNT-11 and DNT-63 was 60 hours and 48 hours, respectively. Both cell lines expressed HLA class I antigens, but were found to be negative for HLA class II. DNT-11 and DNT-63 had a hyperdiploid DNA content. They exhibited anchorage independent growth, and the plating efficiency of DNT-11 and DNT-63 was 0.85% and 1.65%, respectively. These two cell lines may provide a useful tool for studying the biology and immunology of metastatic renal cell carcinoma.
建立并鉴定了两种源自肾细胞癌转移灶的新人类细胞系（DNT - 11和DNT - 63）。它们已在体外传代50次以上，历时4年多。每个细胞系都具有上皮形态。DNT - 11和DNT - 63的细胞周期时间分别为60小时和48小时。两种细胞系均表达HLA I类抗原，但HLA II类抗原呈阴性。DNT - 11和DNT - 63具有超二倍体DNA含量。它们表现出不依赖贴壁生长，DNT - 11和DNT - 63的接种效率分别为0.85%和1.65%。这两种细胞系可能为研究转移性肾细胞癌的生物学和免疫学提供有用的工具。
