Magill Damian J, Shaburova Olga V, Chesnokova Elena N, Pleteneva Elena A, Krylov Victor N, Kulakov Leonid A
Queen's University Belfast, School of Biological Sciences, Medical Biology Centre, Belfast BT9 7BL, Northern Ireland, UK.
Department of Microbiology, Laboratory for Genetics of Bacteriophages, I.I. Mechnikov Research Institute for Vaccines and Sera, Moscow 105064, Russia.
FEMS Microbiol Lett. 2015 May;362(9). doi: 10.1093/femsle/fnv045. Epub 2015 Mar 30.
A complete nucleotide sequence of the new Pseudomonas aeruginosa Luz24likevirus phiCHU was obtained. This virus was shown to have a unique host range whereby it grew poorly on the standard laboratory strain PAO1, but infected 26 of 46 clinical isolates screened, and strains harbouring IncP2 plasmid pMG53. It was demonstrated that phiCHU has single-strand interruptions in its genome. Analysis of the phiCHU genome also suggested that recombination event(s) participated in the evolution of the leftmost portion of the genome, presumably encoding early genes.
获得了新型铜绿假单胞菌Luz24样病毒phiCHU的完整核苷酸序列。该病毒具有独特的宿主范围，在标准实验室菌株PAO1上生长不佳，但能感染46株临床分离株中的26株以及携带IncP2质粒pMG53的菌株。结果表明，phiCHU的基因组存在单链中断。对phiCHU基因组的分析还表明，重组事件参与了基因组最左端部分的进化，这部分可能编码早期基因。
