Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 3, 21 000 Novi Sad, Serbia.
Department of Biotechnology, University of Szeged, Temesvari krt. 62, H-6726 Szeged, Hungary.
Viruses. 2021 Sep 30;13(10):1965. doi: 10.3390/v13101965.
The applicability and safety of bacteriophage Delta as a potential anti- agent belonging to genus (family ) was characterised. Phage Delta belongs to the species , which has been described as a temperate, with sites at the end of the genome. The phage Delta possesses a genome of 45,970 bp that encodes tRNA for proline (Pro), aspartic acid (Asp) and asparagine (Asn) and does not encode any known protein involved in lysogeny formation or persistence. Analysis showed that phage Delta has 182 bp direct terminal repeats at the end of genome and lysogeny was confirmed, neither upon infection at low nor at high multiplicity of infection (MOI). The turbid plaques that appear on certain host lawns can result from bacteriophage insensitive mutants that occur with higher frequency (10). In silico analysis showed that the genome of Delta phage does not encode any known bacterial toxin or virulence factor, determinants of antibiotic resistance and known human allergens. Based on the broad host range and high lytic activity against planktonic and biofilm cells, phage Delta represents a promising candidate for phage therapy.
研究了属（科）噬菌体 Delta 作为一种潜在的抗菌剂的适用性和安全性。噬菌体 Delta 属于温和噬菌体，基因组末端有 个位点。噬菌体 Delta 拥有 45970 个碱基对的基因组，编码 tRNA 用于脯氨酸 (Pro)、天冬氨酸 (Asp) 和天冬酰胺 (Asn)，并且不编码任何已知与溶原形成或持续相关的蛋白质。分析表明，噬菌体 Delta 在基因组末端有 182 个直接的末端重复序列，并且在低感染复数 (MOI) 和高感染复数 (MOI) 下都没有确认溶原。在某些宿主菌苔上出现的混浊斑块可能是由于溶原菌不敏感突变体的出现频率更高（10）。计算机分析表明，Delta 噬菌体的基因组不编码任何已知的细菌毒素或毒力因子、抗生素耐药性决定因素和已知的人类过敏原。基于广泛的宿主范围和对浮游生物和生物膜细胞的高裂解活性，噬菌体 Delta 代表了噬菌体治疗的有前途的候选者。
